초록 ▼

What is described are methods and apparatus for performing a binding assay for an analyte of interest present in a sample. The methods include the steps of: forming a composition containing said sample, an assay-performance-substance which contains a component linked to a label compound capable of c

What is described are methods and apparatus for performing a binding assay for an analyte of interest present in a sample. The methods include the steps of: forming a composition containing said sample, an assay-performance-substance which contains a component linked to a label compound capable of chemiluminescing when triggered, and a plurality of coated magnetic particles capable of specifically binding with the analyte and/or said assay-performance-substance; incubating said composition to form a complex which includes a particle and said labeled component; magnetically collecting said complex at the surface of an electrode; inducing said label to luminesce by contacting it with a trigger, said trigger being formed in-situ by conversion of a precursor molecule upon introduction of electrochemical energy; and measuring the emitted luminescence to measure the presence of the analyte of interest in the sample.

대표청구항 ▼

[ What is claimed is:] [1.] A method for performing a binding assay for an analyte of interest possibly present in a sample comprising the steps of:(a) forming a composition containing(i) said sample(ii) an assay-performance-substance containing at least one component selected from the group consist

[ What is claimed is:] [1.] A method for performing a binding assay for an analyte of interest possibly present in a sample comprising the steps of:(a) forming a composition containing(i) said sample(ii) an assay-performance-substance containing at least one component selected from the group consisting of(1) added analyte of interest or added analogue of said analyte:(2) one or more binding partners of said analyte or said analogue: and(3) one or more reactive components capable of binding with (1) or (2):wherein said assay-performance-substance further contains a label compound linked to at least one of said components, and wherein said label compound is capable of chemiluminescing when triggered, and(iii) a plurality of particles capable of specifically binding with the analyte and/or said assay-performance-substance;(b) incubating said composition to form a complex which includes a particle and said labeled component;(c) subsequently collecting said complex formed by incubation at the surface of an electrode;(d) inducing said label to luminesce by contacting it with a trigger, said trigger being formed in-situ by conversion of a precursor molecule upon introduction of electrochemical energy; and(e) detecting the emitted luminescence to determine the presence in or absence from the sample of the analyte of interest.