초록 ▼

Libraries of unimolecular, double-stranded oligonucleotides on a solid support. These libraries are useful in pharmaceutical discovery for the screening of numerous biological samples for specific interactions between the double-stranded oligonucleotides, and peptides, proteins, drugs and RNA. In a

Libraries of unimolecular, double-stranded oligonucleotides on a solid support. These libraries are useful in pharmaceutical discovery for the screening of numerous biological samples for specific interactions between the double-stranded oligonucleotides, and peptides, proteins, drugs and RNA. In a related aspect, the present invention provides libraries of conformationally restricted probes on a solid support. The probes are restricted in their movement and flexibility using double-stranded oligonucleotides as scaffolding. The probes are also useful in various screening procedures associated with drug discovery and diagnosis. The present invention further provides methods for the preparation and screening of the above libraries.

대표청구항 ▼

[ What is claimed is:] [1.] A method of forming a plurality of diverse unimolecular, double-stranded oligonucleotides on a solid support, said support comprising a surface with a plurality of preselected regions, said method comprising:(a) forming on each of said preselected regions a different firs

[ What is claimed is:] [1.] A method of forming a plurality of diverse unimolecular, double-stranded oligonucleotides on a solid support, said support comprising a surface with a plurality of preselected regions, said method comprising:(a) forming on each of said preselected regions a different first oligonucleotide, each of said first oligonucleotides comprising from 6 to 30 monomers;(b) attaching to the distal end of each of said first oligonucleotides of step (a) a linking group; and(c) forming on the distal end of each of said linking groups a second oligonucleotide, wherein each of said second oligonucleotides is complementary to said first oligonucleotide which is attached within the same preselected region, and wherein said linking groups have sufficient length such that said first and second oligonucleotides form a unimolecular, double-stranded oligonucleotide.