초록 ▼

A method in which a temperature gradient is generated across a "gradient" block, and an apparatus comprising a block across which a temperature gradient can be generated. By setting up such a gradient, multiple reaction mixtures held in wells on the gradient block can be simultaneously run at temper

A method in which a temperature gradient is generated across a "gradient" block, and an apparatus comprising a block across which a temperature gradient can be generated. By setting up such a gradient, multiple reaction mixtures held in wells on the gradient block can be simultaneously run at temperatures which differ only slightly, thereby permitting an optimum temperature for the reaction to be quickly identified. In a preferred embodiment the gradient block is integrated into a thermal cycler used for nucleic acid amplification reactions.

대표청구항 ▼

[ What is claimed is:] [1.] A method for simultaneously reacting a plurality of reaction mixtures in an apparatus including a temperature gradient block and further including first and second uniform temperature blocks, said method comprising the steps of:placing reaction mixtures in a plurality of

[ What is claimed is:] [1.] A method for simultaneously reacting a plurality of reaction mixtures in an apparatus including a temperature gradient block and further including first and second uniform temperature blocks, said method comprising the steps of:placing reaction mixtures in a plurality of reaction wells in said gradient block, said gradient block having first and second opposing end portions, said plurality of reaction mixture wells being formed in said block between said opposing end portions;generating a temperature gradient across said gradient block and between said opposing end portions; andplacing the reaction mixtures in a plurality of reaction wells in said first uniform temperature block; andplacing the reaction mixtures in a plurality of reaction wells in said second uniform temperature block; andgenerating uniform temperatures in said uniform temperature blocks.