초록 ▼

A rapid method for the detection, identification and enumeration of specific respiring microorganisms. The method includes steps of a) passing a microbial sample through a collecting device to capture the cells; b) adding to the collecting device a fluorochrome dye specific for the detection of resp

A rapid method for the detection, identification and enumeration of specific respiring microorganisms. The method includes steps of a) passing a microbial sample through a collecting device to capture the cells; b) adding to the collecting device a fluorochrome dye specific for the detection of respiring cells and allowing the dye to incubate; c) treating the collecting device with a reactive fluorescent antibody which reacts with a target microorganism of interest present in said microbial sample; d) mounting the collecting device for examination by fluorescence microscopy in which a suitable light system is used to excite the fluorochrome dye and fluorescent antibody to fluoresce; and e) quantifying the respiring cells. Alternative embodiments include the use of immunomagnetic beads and other means of cell capture, and employing fluorescent oligonucleotide probes rather than fluorescent antibodies.

대표청구항 ▼

[ We claim:] [1.] A rapid method for the detection, identification and enumeration of respiring microorganisms consisting ofa) passing a microbial sample through a collecting device to obtain a collected sample;b) adding to the collected sample a fluorochrome dye specific for the detection of respir

[ We claim:] [1.] A rapid method for the detection, identification and enumeration of respiring microorganisms consisting ofa) passing a microbial sample through a collecting device to obtain a collected sample;b) adding to the collected sample a fluorochrome dye specific for the detection of respiring cells and allowing the dye to incubate;c) treating the collected sample with a fluorescent antibody which reacts with a microorganism of interest present in said microbial sample, wherein said fluorescent antibody contrasts said fluorochrome dye specific for the detection of respiring cells;d) rinsing away unbound fluorescent antibody;e) fixing said collected sample for examination by fluorescence microscopy in which a suitable light system is used to excite the fluorochrome dye and fluorescent antibody to fluoresce; andf) quantifying respiring microbial cells.