[특허]Rapid self-contained assay format

[미국특허] Rapid self-contained assay format 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	G01N-033/558
출원번호	US-0950331 (1997-10-14)
발명자 / 주소	Bernstein David Childs Mary Ann Trainor William Wier Majorie Gray Erick
출원인 / 주소	Universal Health Watch, Inc.
대리인 / 주소	Foley & Lardner
인용정보	피인용 횟수 : 57 인용 특허 : 16

초록 ▼

A test strip, a device incorporating the test strip and a method for use of the test strip within the device detecting an analyte in a solution or bodily fluid are disclosed. The test strip was three zones, a reaction zone coated with a substance that binds the analyte, a sample zone with a region for addition of the sample of solution or fluid, and a detection region zone with a region coated with detection reagent. An absorbent pad underlies the reaction zone. The reaction zone is a small membrane and is physically in contact on its upper surface with both the sample zone and the detection zone. The test strip is incorporated into a device which includes a sample collection/transferring device, a housing for holding the test strip, and a container for holding solvent solutions at both outer ends of the test strip such that the solvent solutions can come into contact with the outer ends of the test strip when pressure is applied to the ends. The method involves placing a sample onto the strip using the sample collection/transferring device, causing solvent solutions to come into contact with the sample and detection membranes, and observing the test results. The resulting orthogonal buffer flow is an improvement over current methods because it reduces the number of steps and the time required for performance of a sequential assay.

대표청구항 ▼

[ We claim:] [1.] A test strip for use in determining analyte in a test sample, comprising:(a) a reservoir consisting essentially of an absorbent pad;(b) a reaction zone lying directly above the reservoir comprising an upper surface and a lower surface, a first portion of the upper surface containing analyte binding substance and a second portion lacking the substance, and wherein the lower surface is in physical contact with the reservoir pad;(c) an elongated sample zone lying above and in physical contact with a portion of the upper surface of the reaction zone that does not contain analyte binding substance; and(d) an elongated detection reagent zone lying above and in physical contact with the portion of the upper surface of the reaction zone that does not contain analyte binding substance, rein the sample and detection reagent zones lie opposite each other over the reaction zone with their long axes perpendicular to the physical contact between the lower surface of the reaction zone and the reservoir.

이 특허에 인용된 특허 (16) 인용/피인용 타임라인 분석

Gordon Julian (Lake Bluff IL) McMahon Michael E. (Libertyville IL) Ching Shanfun (Vernon Hills IL), Chromatographic test strip for determining ligands or receptors.
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Deoms James H. (Glenarm MD) Mileaf Daryl S. (Jessup MD) LaCour Kevin E. (Jessup MD) Rodriguez ; II Noe E. (Severna Park MD) Leginus Joseph M. (Silver Spring MD) Johnson Scott D. (Elkridge MD) Kapraun, Disposable optical agglutination assay device and method for use.
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Gordon Julian (Arlesheim CHX) Staehelin Theophil (Arlesheim CHX) Towbin Harry (Allschwil CHX), Electrophoretically transferring electropherograms to nitrocellulose sheets for immuno-assays.
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Grubb Anders O. (Lund SEX) Glad Ulla C. (Malmo SEX), Immunoassay with test strip having antibodies bound thereto.
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Zuk Robert F. (Menlo Park CA) Litman David J. (Mountain View CA), Immunochromatographic assay with support having bound “MIP”and second enzyme.
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Chandler Howard M. (Yarmouth ME), Interrupted-flow assay device.
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Eisinger Robert W. (San Diego CA) Khalil Mohammed H. (San Diego CA) Katz David H. (La Jolla CA) Sargeant Robert B. (Ramona CA), Lateral flow, non-bibulous membrane assay protocols.
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Berger Johann (Indianapolis IN) DeLaCroix Fern (Indianapolis IN) Buck Harvey (Indianapolis IN) Schrenk Juergen (Indianapolis IN), Method for determination of a component of a sample.
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Dafforn Geoffrey A. (Los Altos CA) Kurn Nurith (Palo Alto CA) Becker Martin (Palo Alto CA) Ullman Edwin F. (Atherton CA), Multiple port assay device.
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Coleman Patrick F. (Poway CA) Maggio Edward T. (San Diego CA), Orthogonal flow immunoassays and devices.
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Clark Phillip (Stoneham MA), Sample collection and analytical device.
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Mennen, Frederick C.; Freake, Ronald, Self-contained swab cartridge apparatus for detecting occult blood.
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Rosenstein Robert W. (Elliott City MD) Bloomster Timothy G. (Reisterstown MD), Solid phase assay employing capillary flow.
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Giegel Joseph L. (Miami FL) Brotherton Mary M. (Miami FL), Solid phase system for ligand assay.
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Deutsch Marshall E. (Sudbury MA) Mead Louis W. (Lexington MA), Test device and method for its use.
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Kallies Karl-Heinz (Sebnitz DDX), Test device for the detection and determination of glucose.
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Martin Barradine GB; Tracey Gurmin GB, Assay using porosity-reduction to inhibit migration.
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Mink, Ronald W.; Goldstein, Andrew S.; Bohannon, Robert C., Device for collection and assay of oral fluids.
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Mink, Ronald W.; Goldstein, Andrew S.; Bohannon, Robert C., Device for collection and assay of oral fluids.
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Mink,Ronald W.; Goldstein,Andrew S.; Bohannon,Robert C., Device for collection and assay of oral fluids.
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Esfandiari, Javanbakhsh, Dual path immunoassay device.
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Miller, Cary James; Campbell, John Lewis Emerson, Immunosensor system for blood with reduced interference.
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VanDine, Robert W.; Babu, Uma Mahesh; Sambursky, Robert P., In situ lysis of cells in lateral flow immunoassays.
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Babu, Uma Mahesh; Sambursky, Robert P.; VanDine, Robert W., Lateral flow assays.
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Babu, Uma Mahesh; VanDine, Robert W.; Sambursky, Robert P., Lateral flow assays with time delayed components.
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Zhou, Siliang; Rutter, William; Liu, Ning, Lateral flow system and assay.
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Sambursky, Robert P.; VanDine, Robert W.; Babu, Uma Mahesh; Condon, Peter, Method and device for combined detection of viral and bacterial infections.
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Carpenter, Charles, Method and device for detecting analytes in fluids.
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Carpenter, Charles R., Method and device for detecting analytes in fluids.
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Clift, Vaughan L.; Harriman, Kristin L.; Pettys, Richard L.; Headley, David M.; Conerly, Tiffany M., Method for detection of chemicals on a surface.
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Miller, Cary James; Campbell, John Lewis Emerson, Method for measuring an analyte in blood.
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Tyrrell, Steven P.; Grzeda, Barbara R., Whole blood collection device.
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Steven P. Tyrrell, Whole blood collection device and method.
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[미국특허] Rapid self-contained assay format 원문보기

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