초록 ▼

An apparatus for collecting a subpopulation of cells larger than a predetermined size from a collagenase distended organ by circulating fluid through a closed loop system. The closed loop system includes a digesting chamber to liberate cells from the organ, a cell collection chamber, and a filter ad

An apparatus for collecting a subpopulation of cells larger than a predetermined size from a collagenase distended organ by circulating fluid through a closed loop system. The closed loop system includes a digesting chamber to liberate cells from the organ, a cell collection chamber, and a filter adjacent the cell collection chamber for retaining cells larger than the predetermined size. The circulating fluid transports liberated cells through the cell collection chamber to the filter. Subsequently, the cells on the filter are flushed back into the cell collection chamber by a density medium. A tube then withdraws the desired subpopulation of cells from the cell collection chamber.

대표청구항 ▼

[ What I claim as my invention is:] [1.] An apparatus for collecting a subpopulation of cells larger than a predetermined size from a digested organ comprising:a closed loop system including (i) a chamber for digesting the organ to liberate cells, (ii) a cell collection chamber and (iii) a filter ad

[ What I claim as my invention is:] [1.] An apparatus for collecting a subpopulation of cells larger than a predetermined size from a digested organ comprising:a closed loop system including (i) a chamber for digesting the organ to liberate cells, (ii) a cell collection chamber and (iii) a filter adjacent said cell collection chamber for retaining cells larger than the predetermined size;a first pump for circulating a physiologically compatible medium through the closed loop to transport the liberated cells through said cell collection chamber to said filter;a second pump for flushing the cells retained on said filter back into said cell collection chamber with a density medium;a tube for withdrawing the desired subpopulation of cells from said cell collection chamber; anda hollow fiber for containing the withdrawn cells.