초록 ▼

An efficient method for the microfabrication of electronic devices which have been adapted for the analyses of biologically significant analyte species is described. The techniques of the present invention allow for close control over he dimensional features of the various components and layers esta

An efficient method for the microfabrication of electronic devices which have been adapted for the analyses of biologically significant analyte species is described. The techniques of the present invention allow for close control over he dimensional features of the various components and layers established on a suitable substrate. Such control extends to those parts of the devices which incorporate the biological components which enable these devices to function as biological sensors. The materials and methods disclosed herein thus provide an effective means for the mass production of uniform wholly microfabricated biosensors. Various embodiments of the devices themselves are described herein which are especially suited for real time analyses of biological samples in a clinical setting. In particular, the present invention describes assays which can be performed using certain ligand/ligand receptor-based biosensor embodiments. The present invention also discloses a novel method for the electrochemical detection of particular analyte species of biological and physiological significance using an substrate/label signal generating pair which produces a change in the concentration of electroactive species selected from the group consisting of dioxygen and hydrogen peroxide.

대표청구항 ▼

[ What is claimed is:] [1.] A method of detecting the presence and quantity of at least one analyte species in a liquid sample which comprises:(a) contacting a microfabricated biosensor with a liquid sample, said biosensor comprising: (i) a base sensor, (ii) a permselective layer, superimposed over

[ What is claimed is:] [1.] A method of detecting the presence and quantity of at least one analyte species in a liquid sample which comprises:(a) contacting a microfabricated biosensor with a liquid sample, said biosensor comprising: (i) a base sensor, (ii) a permselective layer, superimposed over at least a portion of said base sensor, having a thickness sufficient to exclude substantially molecules with a molecular weight of about 120 or more while allowing the free permeation of molecules with a molecular weight of about 50 or less, and (iii) a biolayer superimposed over at least a portion of said permselective layer and said base sensor, which biolayer comprises a sufficient amount of a bioactive molecule capable of selectively interacting with a particular analyte species, and a support matrix in which said bioactive molecule is incorporated, which matrix is selected from the group consisting of a photoformable proteinaceous mixture, a film-forming latex, and combinations thereof and through which matrix said analyte species may freely permeate and interact with said bioactive molecule; andb) obtaining a measured signal output from said biosensor, from which signal output the presence and quantity of an analyte species may be deduced.