초록 ▼

The present invention provides methods for detecting the presence of or determining the amount of a ligand in a fluid sample. The methods comprise providing a first reagent comprising a sol particle having a detectable physical property bound to the ligand or ligand analogue (in a competitive format

The present invention provides methods for detecting the presence of or determining the amount of a ligand in a fluid sample. The methods comprise providing a first reagent comprising a sol particle having a detectable physical property bound to the ligand or ligand analogue (in a competitive format) or a substance capable of specifically coupling with the ligand (in a sandwich format), providing a second reagent having a detectable physical property comprising a sol particle bound to a substance capable of specifically coupling with the ligand and/or ligand analogue, if present, combining the first reagent, second reagent and the fluid sample and detecting before, during or after the reaction, a change in the physical property of the sol particles, which provides a qualitative or quantitative indication of the ligand in the fluid sample. The reagents couple with one another as a function of the presence of the ligand in the sample to thereby produce a change in the physical property of the sol particles which is related to the degree of coupling of the reagents. The invention also provides a kit for detecting the presence of or determining the amount of a ligand in a fluid sample. The invention further provides immunological complexes having a detectable physical property.

대표청구항 ▼

[ What is claimed:] [1.] A method for detecting the presence of or determining the amount of a ligand in a fluid sample, the method comprising:(a) providing a first reagent comprising a sol particle having a detectable physical property bound (i) to the ligand or (ii) to a substance capable of speci

[ What is claimed:] [1.] A method for detecting the presence of or determining the amount of a ligand in a fluid sample, the method comprising:(a) providing a first reagent comprising a sol particle having a detectable physical property bound (i) to the ligand or (ii) to a substance capable of specifically coupling with the ligand;(b) providing a second reagent comprising a sol particle having a detectable physical property bound to a substance capable of specifically coupling the ligand;(c) combining the first reagent, second reagent and; fluid sample, said second reagent coupling to the first reagent as a function of the presence of the ligand in the sample; and(d) detecting or determining during or after the reaction, a change in the physical property of the sol particles which detection or determination is related to the degree of coupling of the reagents and provides a qualitative or quantitative indication of the ligand in the fluid sample.