초록 ▼

Microfluidic devices are provided for the performance of chemical and biochemical analyses, syntheses and detection. The devices of the invention combine precise fluidic control systems with microfabricated polymeric substrates to provide accurate, low cost miniaturized analytical devices that have

Microfluidic devices are provided for the performance of chemical and biochemical analyses, syntheses and detection. The devices of the invention combine precise fluidic control systems with microfabricated polymeric substrates to provide accurate, low cost miniaturized analytical devices that have broad applications in the fields of chemistry, biochemistry, biotechnology, molecular biology and numerous other fields.

대표청구항 ▼

[ We claim:] [16.] A method of fabricating a microfluidic device, for use with an electroosmotic fluid direction system, comprising:molding a polymeric material to form a substrate having at least one surface, said at least one surface having at least first and second intersecting channels disposed

[ We claim:] [16.] A method of fabricating a microfluidic device, for use with an electroosmotic fluid direction system, comprising:molding a polymeric material to form a substrate having at least one surface, said at least one surface having at least first and second intersecting channels disposed therein, each of said at least first and second intersecting channels having an interior surface, said interior surface having a zeta potential associated therewith, which zeta potential is capable of supporting an electroosmotic mobility of a fluid in said channels of at least 1.times.10.sup.-5 cm.sup.2 V.sup.-1 s.sup.-1, wherein said fluid is from about 1 mM to about 10 mM sodium borate buffer, at a pH of from about 7 to about 9, at least one of said first and second intersecting channels having at least one cross-sectional dimension in a range of from about 0.1 .mu.m to about 500 .mu.m; andoverlaying a cover layer on said at least one surface, said cover layer enclosing said first and second intersecting channels, and wherein said substrate and said cover layer together comprise at least three ports disposed therein, each of said at least three ports being in fluid communication with first and second termini of said first channel and at least one terminus of said second channel.