초록 ▼

A rapid detection of ATP using dried luciferase/luciferin reagent. A liquid that may include an extraction reagent is applied to the testing surface, such as a counter top. A sampling device having a handle and an absorbent tip is wiped across the testing surface. The absorbent tip absorbs the sampl

A rapid detection of ATP using dried luciferase/luciferin reagent. A liquid that may include an extraction reagent is applied to the testing surface, such as a counter top. A sampling device having a handle and an absorbent tip is wiped across the testing surface. The absorbent tip absorbs the sample. The sample is then placed in a counting tube. Luciferase/luciferin reagent is immobilized on the absorbent tip of the sampling device or in the bottom of a counting tube. In either case, the liquid rehydrates the luciferase/luciferin reagent allowing ATP present in the sample to react with the luciferase. Light produced by the reaction is measured with a luminometer.

대표청구항 ▼

[ What is claimed is:] [1.] A method of measuring ATP using a sampling device having an absorbent tip attached to a handle, and a counting tube containing dried luciferase/luciferin reagent comprising:adding an adequate amount of a liquid to the test surface to wet the surface,rubbing the test surfa

[ What is claimed is:] [1.] A method of measuring ATP using a sampling device having an absorbent tip attached to a handle, and a counting tube containing dried luciferase/luciferin reagent comprising:adding an adequate amount of a liquid to the test surface to wet the surface,rubbing the test surface with the absorbent tip of the sampling device to absorb the liquid into the absorbent tip,placing the sampling device into the counting tube so that the absorbent tip is placed at the bottom of the counting tube in order to rehydrate the dried luciferase/luciferin in the tube;placing the counting tube including the sampling device in a luminometer; andmeasuring the amount of light produced by the reaction of luciferase and ATP in the sample.