초록

L-tertiary-Leucine and L-phosphinothricine are obtainable by transamination of the corresponding keto acids as a precursor in the presence of amino acids as amino group donors. The reaction is preferably carried out with microorganisms or their transaminases.

대표청구항 ▼

[ We claim:] [1.] A process for preparing L-phosphinothricine, which comprises incubating an .alpha.-keto acid selected from (3-carboxy-3-oxo-propyl)-methylphosphinic acid and a salt of (3-carboxy-3-oxo-propyl)-methylphosphinic acid in the presence of:(i) an L-amino acid as an amino group donor, and

[ We claim:] [1.] A process for preparing L-phosphinothricine, which comprises incubating an .alpha.-keto acid selected from (3-carboxy-3-oxo-propyl)-methylphosphinic acid and a salt of (3-carboxy-3-oxo-propyl)-methylphosphinic acid in the presence of:(i) an L-amino acid as an amino group donor, and(ii) a microorganism which transaminates the .alpha.-keto acid, or transaminase enzyme of said microorganism, wherein:(A) said microorganism is selected from the genera Paracoccus, Alcaligenes, Pseudomonas, Serratia, Agrobacterium, Streptomyces, Escherichia, or Enterobacter, or is a soil isolate selected from the group consisting of soil isolate DSM 4113, soil isolate DSM 4117, and soil isolate DSM 4118; and(B) the genome of said microorganism contains a transformed or mutated gene such that said microorganism's ability to transaminate said .alpha.-keto acid is enhanced, whereinsaid transformed or mutated gene is derived from said genera or said soil isolate.