초록 ▼

A method for purifying protein containing histidine residues using immobilized metal, affinity chromatography. The hydrophilic index of the histidine residues is determined (HI). If the HI is at least 2 the pH of the solution containing the protein is adjusted to about 6.75 to 7.2 and applied to the

A method for purifying protein containing histidine residues using immobilized metal, affinity chromatography. The hydrophilic index of the histidine residues is determined (HI). If the HI is at least 2 the pH of the solution containing the protein is adjusted to about 6.75 to 7.2 and applied to the IMAC column such that the protein binds to the column.

대표청구항 ▼

[ We claim:] [1.] A method for purifying a protein contained in a solution using Immobilized Metal Ion Affinity Chromatography (IMAC) comprising:(a) sequencing the protein and determine if the protein contains one or more histidine residues;(b) determining the hydrophilicity index (HI) and/or the So

[ We claim:] [1.] A method for purifying a protein contained in a solution using Immobilized Metal Ion Affinity Chromatography (IMAC) comprising:(a) sequencing the protein and determine if the protein contains one or more histidine residues;(b) determining the hydrophilicity index (HI) and/or the Solvent-Exposed Surface Area (SESA) of the histidine residues of said protein;(c) adjusting the pH of the solution to about 6.75 to 7.2 if the HI of at least one of the histidine residues is at least 2 and/or the SESA of at least one histidine residue of the protein is at least about 3.5 (.ANG.).sup.2 ;(c) applying the solution to an IMAC column, so that the protein binds to the column; and(d) eluting the protein from the column.