초록 ▼

The present invention relates to devices and methods for the collection, storage, and purification of nucleic acids, such as DNA or RNA, from fluid samples for subsequent genetic characterization, primarily by conventional amplification methods. The present invention can be used to collect, store, o

The present invention relates to devices and methods for the collection, storage, and purification of nucleic acids, such as DNA or RNA, from fluid samples for subsequent genetic characterization, primarily by conventional amplification methods. The present invention can be used to collect, store, or purify nucleic acids from a treated whole blood source that has naturally occurring nucleic acid amplification inhibitors present, as well as added blood stabilization components that also inhibit nucleic acid amplification. More importantly, these nucleic acids can be released after collection or storage in a manner that enables them to be amplified by conventional techniques such as polymerase chain reaction. In particular, an absorbent material that does not bind nucleic acids irreversibly is impregnated with a chaotropic salt. A biological source sample is contacted with the impregnated absorbent material. Any nucleic acids present in the biological source can be either eluted or resolubilized off the absorbent material.

대표청구항 ▼

[ We claim:] [1.] A method for collecting and storing nucleic acids from a whole blood source having naturally occurring nucleic acid amplification inhibitors and an added blood stabilization agent selected from the group consisting of EDTA, heparin and citrate, comprising:a) contacting said whole b

[ We claim:] [1.] A method for collecting and storing nucleic acids from a whole blood source having naturally occurring nucleic acid amplification inhibitors and an added blood stabilization agent selected from the group consisting of EDTA, heparin and citrate, comprising:a) contacting said whole blood source with an adsorbent material that does not bind to nucleic acids and which has a chaotropic salt impregnated about the adsorbent material; andb) allowing the treated whole blood source to be absorbed onto or dried on the absorbent material for storage.