초록

The nucleic acid amplification reactions of the present invention are conducted isothermally, using non-thermal denaturation procedures, such as occurs in chemical- or electrostatic-based denaturation. Embodiments are set forth for automated forms of the claimed procedures.

대표청구항 ▼

[ What is claimed is:] [1.] An amplification device for target nucleic acids comprising a reaction chamber and at least one supply chamber, wherein amplicons complementary to a target nucleic acid segment of the target nucleic acid are generated in a reaction chamber within the device and form duple

[ What is claimed is:] [1.] An amplification device for target nucleic acids comprising a reaction chamber and at least one supply chamber, wherein amplicons complementary to a target nucleic acid segment of the target nucleic acid are generated in a reaction chamber within the device and form duplex amplicons between complementary amplicons, and wherein denaturation of the duplex amplicons is effected in the reaction chamber by a chemical denaturant or an electrostatic process wherein the device further comprises:an electric field generating device effective to create a field at a reaction chamber of the device such that the strands of amplicons in that chamber separate, wherein the amplicons are each formed from first primer and a second primer, where the first primers have one charge and the second primers have the opposite charge.