초록 ▼

Specific epitopic regions of thyroid peroxidase (TPO), a thyroid specific membrane autoantigen, have been identified within amino acid residues 456 to 933, 517 to 630, and 633 to 933 of the protein (SEQ ID NO:1), with at least one distinct binding region within TPO located from amino acid residues 5

Specific epitopic regions of thyroid peroxidase (TPO), a thyroid specific membrane autoantigen, have been identified within amino acid residues 456 to 933, 517 to 630, and 633 to 933 of the protein (SEQ ID NO:1), with at least one distinct binding region within TPO located from amino acid residues 592 to 613 (SEQ ID NO:3). The identification and production of these localized epitopes/epitopic regions provide specific diagnostic reagents for autoimmune thyroid disease.

대표청구항 ▼

[ We claim:] [1.] A method of screening for presence of autoantibody to thyroid peroxidase in a sample from a patient comprising the steps of:(a) providing the sample to be screened from a patient;(b) providing a thyroid peroxidase reagent comprising a substantially pure thyroid peroxidase epitopic

[ We claim:] [1.] A method of screening for presence of autoantibody to thyroid peroxidase in a sample from a patient comprising the steps of:(a) providing the sample to be screened from a patient;(b) providing a thyroid peroxidase reagent comprising a substantially pure thyroid peroxidase epitopic peptide which consists of amino acid residue sequence SEQ ID NO:3 (residues 592 to 613 of SEQ ID NO:1);(c) contacting the sample with the thyroid peroxidase reagent under conditions favorable for specific binding of the autoantibody present in the sample to the epitopic peptide; and(d) detecting specific binding of the autoantibody in the sample to the epitopic peptide as indicative of the presence of the autoantibody to thyroid peroxidase in the sample from the patient.