초록 ▼

An apparatus in the form of a test strip is useful for determining the presence of one or more components in a fluid sample. The test strip can be used by itself or in conjunction with an associated housing assembly. The test strip provides a rapid volume, timing and temperature independent visually

An apparatus in the form of a test strip is useful for determining the presence of one or more components in a fluid sample. The test strip can be used by itself or in conjunction with an associated housing assembly. The test strip provides a rapid volume, timing and temperature independent visually readable strip for the semi-quantitative detection of a component, such as an analyte.

대표청구항 ▼

[ What is claimed is:] [1.] A semiquantitative method for determining the presence of an analyte in a sample fluid, comprisingexposing a portion of a test strip apparatus to said sample fluid to be tested, wherein said test strip apparatus comprises:a conjugate pad comprising a diffusible labelled f

[ What is claimed is:] [1.] A semiquantitative method for determining the presence of an analyte in a sample fluid, comprisingexposing a portion of a test strip apparatus to said sample fluid to be tested, wherein said test strip apparatus comprises:a conjugate pad comprising a diffusible labelled first binding partner that binds with said analyte;a first area comprising an immobilized second binding partner that competes with said analyte to bind with said diffusible labelled first binding partner; anda second area comprising an immobilized third binding partner that binds with said diffusible labelled first binding partner,located in sequence in a capillary fluid flow direction in said test strip apparatus;allowing said analyte in said sample fluid to migrate up the test strip apparatus by capillary action; andreading a result of said test strip by comparing relative label intensities in said first area and said second area,wherein a greater relative label intensity in said second area as compared to said first area indicates the presence of said analyte in said sample fluid in an amount greater than a value determined by the amounts of said first and second binding partners and a lesser relative intensity in said second area as compared to said first area indicates the presence of said analyte in said sample fluid in an amount less than a value determined by the amounts of said first and second binding partners.