초록 ▼

The present invention provides a microfabricated sensor and a method capable of rapid simultaneous measurement of multiple analytes in a fluid sample. The sensor is inexpensive, disposable and portable, and requires only microliters of sample, a particular advantage with precious fluids such as bloo

The present invention provides a microfabricated sensor and a method capable of rapid simultaneous measurement of multiple analytes in a fluid sample. The sensor is inexpensive, disposable and portable, and requires only microliters of sample, a particular advantage with precious fluids such as blood. The sensor utilizes diffusion between layered laminar streams rather than side by side streams. This allows multiple side by side channels for simultaneous detection of multiple analytes. In the sensor, a sample stream and a carrier stream flow in layers, one on top of the other, and one or more reagents are introduced to the bottom of the carrier stream through either a fluid or a solid reagent inlet. The reagent contains reagent particles which, in the presence of the analyte, have a detectable change in a property. The analyte diffuses into the carrier stream where it interacts with reagent particles and is detected by optical, electrochemical or other devices.

대표청구항 ▼

[ I claim:] [1.] A diffusion based sensor, comprising:a sample inlet;an outlet;a laminar flow channel connected to said sample inlet and said outlet, whereby a laminar flow of sample fluid can be established between said sample inlet and said outlet;a carrier inlet connecting with the bottom of said

[ I claim:] [1.] A diffusion based sensor, comprising:a sample inlet;an outlet;a laminar flow channel connected to said sample inlet and said outlet, whereby a laminar flow of sample fluid can be established between said sample inlet and said outlet;a carrier inlet connecting with the bottom of said laminar flow channel between said sample inlet and said outlet, whereby a laminar flow of carrier fluid can be established between said carrier inlet and said outlet, said carrier fluid forming a layer below said sample fluid;said channel being greater in depth at said carrier inlet than at said sample inlet;a first reagent inlet connecting with the bottom of said channel between said carrier inlet and said outlet.