초록 ▼

A self-addressable, self-assembling microelectronic device is designed and fabricated to actively carry out and control multi-step and multiplex molecular biological reactions in microscopic formats. These reactions include nucleic acid hybridizations, antibody/antigen reactions, diagnostics, and bi

A self-addressable, self-assembling microelectronic device is designed and fabricated to actively carry out and control multi-step and multiplex molecular biological reactions in microscopic formats. These reactions include nucleic acid hybridizations, antibody/antigen reactions, diagnostics, and biopolymer synthesis. The device can be fabricated using both microlithographic and micro-machining techniques. The device can electronically control the transport and attachment of specific binding entities to specific micro-locations. The specific binding entities include molecular biological molecules such as nucleic acids and polypeptides. The device can subsequently control the transport and reaction of analytes or reactants at the addressed specific micro-locations. The device is able to concentrate analytes and reactants, remove non-specifically bound molecules, provide stringency control for DNA hybridization reactions, and improve the detection of analytes. The device can be electronically replicated.

대표청구항 ▼

[ What is claimed is:] [1.] A method for electronically controlling nucleic acid hybridization, comprising the steps of:connecting multiple locations to an electrical source;contacting a plurality of specific nucleic acids with target nucleic acids, wherein said specific nucleic acids are attached t

[ What is claimed is:] [1.] A method for electronically controlling nucleic acid hybridization, comprising the steps of:connecting multiple locations to an electrical source;contacting a plurality of specific nucleic acids with target nucleic acids, wherein said specific nucleic acids are attached to said locations; andplacing at least one of said locations at a negative potential for a sufficient time, wherein a non-specific nucleic acid sequence to said target nucleic acid but not a specific nucleic acid sequence from said plurality of nucleic acids is removed from said target nucleic acid.