초록 ▼

The present invention provides a miniaturized integrated nucleic acid diagnostic device and system. The device of the invention is generally capable of performing one or more sample acquisition and preparation operations, in combination with one or more sample analysis operations. For example, the d

The present invention provides a miniaturized integrated nucleic acid diagnostic device and system. The device of the invention is generally capable of performing one or more sample acquisition and preparation operations, in combination with one or more sample analysis operations. For example, the device can integrate several or all of the operations involved in sample acquisition and storage, sample preparation and sample analysis, within a single integrated unit. The device is useful in a variety of applications, and most notably, nucleic acid based diagnostic applications and de novo sequencing applications.

대표청구항 ▼

[ What is claimed is:] [1.] A miniature analytical device, comprising:a body having at least first, second and third chambers in fluid communication within said body;said first chamber having disposed therein at least one reagent for amplifying a nucleic acid segment within a sample,said second cham

[ What is claimed is:] [1.] A miniature analytical device, comprising:a body having at least first, second and third chambers in fluid communication within said body;said first chamber having disposed therein at least one reagent for amplifying a nucleic acid segment within a sample,said second chamber for fragmenting said nucleic acid segmentand said third chamber having an array of nucleic acid probes disposed therein, said array including a plurality of different nucleic acid sequences coupled to a surface of a single substrate, each of said plurality of different nucleic acid sequences being coupled to said surface in a different, known location. [26.] A method of analyzing a nucleic acid test sample in a miniature analytic device having first, second and third chambers in fluid communication, comprising:amplifying the nucleic acid test sample in a first chamber;transporting the nucleic acid test sample from the first chamber to a second chamber;fragmenting the nucleic acid test sample in the second chamber;transporting the nucleic acid test sample from the second chamber to a third chamber; andhybridizing the nucleic acid test sample to a nucleic acid probe under hybridization conditions in the third chamber.