Disclosed are compositions with tethered growth effector molecules, and methods of using these compositions for growing cells and tissues. Growth effector molecules, including growth factors and extracellular matrix molecules, are flexibly tethered to a solid substrate. The compositions can be used
Disclosed are compositions with tethered growth effector molecules, and methods of using these compositions for growing cells and tissues. Growth effector molecules, including growth factors and extracellular matrix molecules, are flexibly tethered to a solid substrate. The compositions can be used either in vitro or in vivo to grow cells and tissues. By tethering the growth factors, they will not diffuse away from the desired location. By making the attachment flexible, the growth effector molecules can more naturally bind to cell surface receptors. A significant feature of these compositions and methods is that they enhance the biological response to the growth factors. The new method also offers other advantages over the traditional methods, in which growth factors are delivered in soluble form: (1) the growth factor is localized to a desired target cell population; (2) significantly less growth factor is needed to exert a biologic response. This method can be used as a means of enhancing the therapeutic use of growth factors in vivo and of creating surfaces which will enhance in vitro growth of difficult-to-grow cells such as liver cells.
대표청구항▼
[ We claim:] [9.] A method for growing eukaryotic cells comprising(a) bringing into contact the cells and a composition comprisinga biocompatible solid substrate,biocompatible branched water soluble polymeric tethers comprising a polymeric material selected from the group consisting of polyethylene
[ We claim:] [9.] A method for growing eukaryotic cells comprising(a) bringing into contact the cells and a composition comprisinga biocompatible solid substrate,biocompatible branched water soluble polymeric tethers comprising a polymeric material selected from the group consisting of polyethylene oxide, polyvinyl alcohol, polyhydroxyalkyl (meth)acrylate, polyacrylamide, and starches, andgrowth effector molecules,wherein one end of each tether is covalently linked to the substrate, each tether is able to covalently link more than one growth effector molecule, each growth effector molecule is covalently linked to a distal end of a tether so that the growth effector molecule cannot be internalized by cells attached to the substrate, and the growth effector molecules are attached to the substrate in a concentration effective to enhance the rate of target cell growth over the rate of target cell growth with soluble growth effector molecules and growth effector molecules adsorbed to a substrate, without internalization of the molecules; and(b) maintaining the contacting cells and composition under conditions and for a time sufficient to cause the cells to grow,wherein the substrate is selected from the group consisting of glasses, metals, polystyrenes, polyethylene vinyl acetates, polypropylenes, polymethacrylates, polyacrylates, polyethylenes, polyethylene oxides, polysilicates, polycarbonates, polytetrafluoroethylene, fluorocarbons, nylon, silicon rubber, polyanhydrides, polyglycolic acids, polyhydroxyacids, polyesters, polycapralactone, polyhydroxybutyrate, polyphosphazenes, polyorthoesters, polyurethanes, and combinations thereof.
Asensio Javier (Tgerwilen CHX) Hayman Richard (Frauenfeld CHX), Assembly of several layers having one or more reinforcing layers and fiber reinforced plastic article produced therefrom.
Clapper David L. (Shorewood MN) Hu Wei-Shou (Falcon Heights MN), Cell culture support containing a cell adhesion factor and a positively-charged molecule.
Yannas Ioannis V. (Newton Center MA) Burke John F. (Belmont MA) Gordon Philip L. (Lexington MA) Huang Chor (Avon Lake OH), Multilayer membrane useful as synthetic skin.
Marans Nelson S. (Silver Spring MD) Pulford Stephen M. (Baltimore MD), Water soluble linear polyethyleneimine derivative from water-insoluble polyethyleneimine, polyvinyl alcohol and aldehyde.
Wall, Wesley James; Wall, Calvin Lee Michael; Smart, Ryan George, Method of manufacture of a liquid pesticide containing copper and a liquid pesticide containing copper.
Borenstein, Jeffrey T.; Weinberg, Eli; Orrick, Brian K.; Pritchard, Eleanor M.; Barnard, Edward J.; Krebs, Nicholas J.; Marentis, Theodore; Vacanti, Joseph P.; Kaazempur-Mofrad, Mohammad Reza, Micromachined bilayer unit for filtration of small molecules.
Borenstein, Jeffrey T.; Weinberg, Eli; Orrick, Brian K.; Pritchard, Eleanor M.; Barnard, Edward J.; Krebs, Nicholas J.; Marentis, Theodore; Vacanti, Joseph P.; Kaazempur-Mofrad, Mohammad Reza, Micromachined bilayer unit of engineered tissues.
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