초록 ▼

Disclosed are materials and methods for performing multiplex assays for nucleic acids, in which a transponder is associated with the bead(s) forming the solid phase used in the assay, nucleic acid probes are bound to the surface of the particles, and data concerning the assay is encoded on the trans

Disclosed are materials and methods for performing multiplex assays for nucleic acids, in which a transponder is associated with the bead(s) forming the solid phase used in the assay, nucleic acid probes are bound to the surface of the particles, and data concerning the assay is encoded on the transponder. A dedicated read/write device is used to remotely encode or read the data.

대표청구항 ▼

[ I claim:] [1.] A method of detecting a target nucleic acid sequence in a sample, comprising the steps of:(a) providing a solid phase comprising particles having transponders, the transponders having memory elements, the particles having a nucleic acid probe attached to a surface of the solid phase

[ I claim:] [1.] A method of detecting a target nucleic acid sequence in a sample, comprising the steps of:(a) providing a solid phase comprising particles having transponders, the transponders having memory elements, the particles having a nucleic acid probe attached to a surface of the solid phase particles, the probe having a sequence complementary to a target sequence, the transponders having an index number encoded in the memory element;(b) contacting the solid phase with a sample to form a sample mixture;(c) increasing the amount of the target nucleic acid subjected to analysis by PCR amplification, using at least one oligonucleotide primer which is not immobilized on the solid phase, said PCR amplification comprising at least one cycle of:(1) denaturation of nucleic acids in the sample mixture;(2) hybridization of nucleic acids in the sample mixture;(3) chain extension with DNA polymerase;(d) analyzing the solid phase to detect the presence of a label indicative of binding of the target nucleic acid; and(e) decoding the data encoded on transponders using the dedicated read/write scanner to identify the class of transponders to which analytes are bound.