초록 ▼

This invention is directed to a method for producing somatic embryos on plant tissue. Furthermore, somatic embryos may be produced using explants obtained from a broad range of plant species, and using either juvenile or mature tissues. The method involves obtaining a stock tissue culture plantlet b

This invention is directed to a method for producing somatic embryos on plant tissue. Furthermore, somatic embryos may be produced using explants obtained from a broad range of plant species, and using either juvenile or mature tissues. The method involves obtaining a stock tissue culture plantlet by exposing the plant tissue to a medium comprising salts, vitamins and an energy source; preparing an explant from the stock tissue culture plantlet; transferring the explant to a proliferation medium comprising salts, vitamins, an energy source and at least one growth regulator for a period of time sufficient to produce a callused explant; and transferring the callused explant to a medium comprising salts, vitamins, an energy source and at least two growth regulators for a period of time sufficient to produce somatic embryos. Following this method somatic embryos are produce in significantly less time that observed using other somatic embryogenesis protocols. Plants produced from the somatic embryos are easily prepared. Since somatic embryos can be obtained from mature tissues, specific plants of agronomic importance comprising desirable traits, may now be clonally propagated. Furthermore, somatic embryos produced following the method of this invention may be used for the preparation of synthetic seed.

대표청구항 ▼

[ What is claimed is:] [1.] A method for producing somatic embryos on potato plant tissue obtained from a non-juvenile plant comprising:i) obtaining a stock tissue culture plantlet by exposing potato plant tissue obtained from a non-juvenile plant to a growth regulator-free medium comprising salts,

[ What is claimed is:] [1.] A method for producing somatic embryos on potato plant tissue obtained from a non-juvenile plant comprising:i) obtaining a stock tissue culture plantlet by exposing potato plant tissue obtained from a non-juvenile plant to a growth regulator-free medium comprising salts, vitamins and an energy source;ii) preparing an explant from the stock tissue culture plantlet;iii) transferring the explant to a proliferation medium comprising salts, vitamins, an energy source and at least one growth regulator selected from the group consisting of a cytokinin or a cytokinin-like compound; and Indole acetic acid combined with said cytokinin or cytokinin-like compound, for about 3 days to less than 4 weeks, sufficient to produce a callused explant; andiv) transferring the callused explant to a medium comprising salts, vitamins, an energy source and at least two growth regulators comprising at least one auxin and at least one cytokinin or cytokinin-like compound for a period of time sufficient to produce somatic embryos.