초록 ▼

A method for cleaning and disinfecting a surface at least partly covered by a biofilm layer comprising the steps of contacting the biofilm with a cleaning composition comprising one or more hydrolases, e.g. a hydrolytic enzyme produced by a strain of the fungus Aspergillus aculeatus, in an amount ef

A method for cleaning and disinfecting a surface at least partly covered by a biofilm layer comprising the steps of contacting the biofilm with a cleaning composition comprising one or more hydrolases, e.g. a hydrolytic enzyme produced by a strain of the fungus Aspergillus aculeatus, in an amount effective for either fully or partly removing or releasing the biofilm layer from the surface; and contacting the biofilm with a bactericidal disinfecting composition comprising an oxidoreductase such as an oxidase, a peroxidase or a laccase, in an amount effective for killing the living bacterial cells present in the biofilm. In particular, a disinfecting composition comprising laccase at concentration between about 0.01 to about 1000 mg protein/ml composition and an oxidation enhancer such as methyl syringate.

대표청구항 ▼

[ What is claimed is:] [1.] A method for cleaning and disinfecting a surface at least partially covered by a biofilm layer, the method comprising contacting the biofilm, sequentially or simultaneously, with:(a) a cleaning composition comprising one or more hydrolases in an amount effective for at le

[ What is claimed is:] [1.] A method for cleaning and disinfecting a surface at least partially covered by a biofilm layer, the method comprising contacting the biofilm, sequentially or simultaneously, with:(a) a cleaning composition comprising one or more hydrolases in an amount effective for at least partially releasing the biofilm layer from the surface; and(b) a disinfecting composition comprising (i) a laccase at a concentration between about 0.01 to about 1000 mg protein/ml composition and (ii) an oxidation enhancer, wherein said enhancer has the formula [ STR 3 ] wherein (i) A is selected from the group consisting of --CO--D and --SO.sub.2 --D, wherein D is --H, --OH, --R, or --OR; R being a C.sub.1 -C.sub.16 alkyl; and(ii) B and C are the same or different and have the formula C.sub.m H.sub.2m+1, wherein 1.ltoreq.m.ltoreq.5.