초록 ▼

The present invention is generally directed to microfluidic systems and methods of using such systems in the determination of the nucleotide sequence of target nucleic acid sequences (referred to herein as the "target"). In particular, the present invention provides methods and systems for determini

The present invention is generally directed to microfluidic systems and methods of using such systems in the determination of the nucleotide sequence of target nucleic acid sequences (referred to herein as the "target"). In particular, the present invention provides methods and systems for determining the relative positions within a target nucleic acid sequence that are occupied by a given nucleotide, e.g., A, T, G or C, by separating mixtures of nested sets of fragments of the target nucleic acid, which sets each include fragments that terminate in a different given nucleotide.

대표청구항 ▼

[ What is claimed is:] [1.] A method of mixing and separating nucleic acids, the method comprising:mixing a plurality of sets of nested nucleic acid fragments in a mixing channel;flowing the resulting nucleic acid fragment mixture to a fluidly coupled separated channel comprising a separation matrix

[ What is claimed is:] [1.] A method of mixing and separating nucleic acids, the method comprising:mixing a plurality of sets of nested nucleic acid fragments in a mixing channel;flowing the resulting nucleic acid fragment mixture to a fluidly coupled separated channel comprising a separation matrix; and,separating the nucleic acid fragment mixture by flowing the mixture through the separation channel, thereby providing separated nucleic acid fragments.