초록 ▼

A method for introgressing soybean cyst nematode resistance into elite soybean germplasm is disclosed. The method involves using genetically mapped loci associated with soybean cyst nematode resistance for marker-assisted selection during introgression of soybean cyst nematode resistance into elite

A method for introgressing soybean cyst nematode resistance into elite soybean germplasm is disclosed. The method involves using genetically mapped loci associated with soybean cyst nematode resistance for marker-assisted selection during introgression of soybean cyst nematode resistance into elite soybean germplasm. Also disclosed are a method for confirming selection for soybean cyst nematode resistance; quantitative trait loci associated with soybean cyst nematode resistance; and soybean lines bred to be resistant to soybean cyst nematode infestation. Also disclosed is a method for using the mapped markers for positional cloning of soybean cyst nematode resistance genes.

대표청구항 ▼

[ What is claimed is:] [1.] A method of selecting a first plant by marker assisted selection of a quantitative trait locus ("QTL") associated with soybean cyst nematode resistance, the method comprising the steps of:(i) detecting by hybridization a first nucleic acid from the first plant which is ge

[ What is claimed is:] [1.] A method of selecting a first plant by marker assisted selection of a quantitative trait locus ("QTL") associated with soybean cyst nematode resistance, the method comprising the steps of:(i) detecting by hybridization a first nucleic acid from the first plant which is genetically linked to a locus corresponding to a marker selected from the group consisting of pA85a, php02302a, php02340a, pk400a, pT155a, pBLT24a, pBLT65a, php05180a, pSAC3a, pA1116, php05266a, php02298b, pA664a, pA63a, php02366a, php02361a, php05354a, php05219a, pK69a, pL50c, pK18a, pA567a, pA407a, pA404b, pA226a, pA715a, pK24a, pB157b, php02275a, php05278a, php05240c, pBLT49a, pK79a, and php03488a and,(ii) selecting the first plant comprising the first nucleic acid, thereby selecting the QTL. [15.] A soybean cyst nematode resistant plant selected by a method of claim 1, the method comprising detecting by hybridization a first nucleic acid from the first plant which is genetically linked to a locus corresponding to a marker selected from the group consisting of pA85a, php02302a, php02340a, pk400a, pT155a, pBLT24a, pBLT65a, php05180a, pSAC3a, pA1116, php05266a, php02298b, pA664a, pA63a, php02366a, php02361a, php05354a, php05219a, pK69a, pL50c, pK18a, pA567a, pA407a, pA404b, pA226a, pA715a, pK24a, pB157b, php02275a, php05278a, php05240c, pBLT49a, pK79a, and php03488a. The plant of claim 15, wherein the QTL conferring resistance maps to a linkage group selected from the group consisting of A2, C1, G, M, L25 and L26. A method of identifying a quantitative trait locus ("QTL") associated with soybean cyst nematode resistance in a plant, the method comprising detecting by hybridization a first locus genetically linked to the QTL, wherein the first locus corresponds to a marker selected the group consisting of: pA85a, php02302a, php02340a, pk400a, pT155a, pBLT24a, pBLT65a, php05180a, pSAC3a, pA1116, php05266a, php02298b, pA664a, pA63a, php02366a, php02361a, php05354a, php05219a, pK69a, pL50c, pK18a, pA567a, pA407a, pA404b, pA226a, pA715a, pK24a, pB157b, php02275a, php05278a, php05240c, pBLT49a, pK79a, and php03488a, whereby detecting the locus linked to the QTL provides an indication that the QTL is present in the plant. The method of claim 17, further comprising detecting a second locus genetically linked to the QTL, wherein the second locus corresponds to a marker selected the group consisting of: pA85a, php02302a, php02340a, pk400a, pT155a, pBLT24a, pBLT65a, php05180a, pSAC3a, pA1116, php05266a, php02298b, pA664a, pA63a, php02366a, php02361a, php05354a, php05219a, pK69a, pL50c, pK18a, pA567a, pA407a, pA404b, pA226a, pA715a, pK24a, pB157b, php02275a, php05278a, php05240c, pBLT49a, pK79a, and php03488a. The method of claim 17, further comprising detecting a second locus genetically linked to the QTL, wherein the second locus corresponds to a marker selected the group consisting of: pA85a, php02302a, php02340a, pk400a, pT155a, pBLT24a, pBLT65a, php05180a, pSAC3a, pA1116, php05266a, php02298b, pA664a, pA63a, php02366a, php02361a, php05354a, php05219a, pK69a, pL50c, pK18a, pA567a, pA407a, pA404b, pA226a, pA715a, pK24a, pB157b, php02275a, php05278a, php05240c, pBLT49a, pK79a, and php03488a, wherein the first and second loci flank the QTL.