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Method of detecting nucleic acids 원문보기

IPC분류정보
국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판)
  • C12Q-001/68
  • C12P-019/34
출원번호 US-0056927 (1998-04-08)
발명자 / 주소
  • Fodor Stephen P. A.
  • Solas Dennis W.
  • Dower William J.
출원인 / 주소
  • Affymetrix, Inc.
대리인 / 주소
    Cushman Darby & Cushman IP Group of Pillsbury Madison & Sutro
인용정보 피인용 횟수 : 142  인용 특허 : 42

초록 ▼

The present invention provides methods and apparatus for sequencing, fingerprinting and mapping biological macromolecules, typically biological polymers. The methods make use of a plurality of sequence specific recognition reagents which can also be used for classification of biological samples, and

대표청구항 ▼

[What is claimed is:] [1.](a) providing a substrate having at least 100 different polynucleotides in known locations at a density of at least 50 different polynucleotides per square centimeter,(b) hybridizing a first nucleic acid sample to the substrate to obtain a first hybridization pattern,(c) hy

이 특허에 인용된 특허 (42)

  1. Stavrianopoulos Jannis (New York NY) Rabbani Elazar (New York NY) Abrams Samuel B. (New York NY) Wetmur James G. (Scardsdale NY), Analyte detection by means of energy transfer.
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  2. Southern Edwin (Oxford GB2), Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays.
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  4. Dattagupta Nanibhushan (West Haven CT) Rae Peter M. M. (Hamden CT) Rabin Daniel U. (Branford CT) Huguenel Edward D. (Guilford CT), Assay for nucleic acid sequences in an unpurified sample.
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  5. Taub Floyd (Rockville MD), Assay for nucleic acid sequences, particularly genetic lesions, using interactive labels.
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  8. Dattagupta, Nanibhushan; Crothers, Donald M., Coupling of nucleic acids to solid support by photochemical methods.
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  9. Chehab Farid F. (San Francisco CA), DNA detection by color complementation.
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  10. Smith Lloyd M. (South Pasadena CA) Fung Steven (Palo Alto CA), Deoxyribonucleoside phosphoramidites in which an aliphatic amino group is attached to the sugar ring and their use for t.
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  11. Longiaru Mathew (West Orange NJ) Silver Sheryl B. (Fort Lee NJ) Sulzinski Michael A. (Spring Valley NY), Detection of chlamydia trachomatis by polymerase chain reaction using biotin labelled lina primers and capture probes.
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  15. Ullman Edwin F. (Atherton CA), Fluorescent multiparameter particle analysis.
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  20. Koester Hubert (Concord MA) Coull James M. (Acton MA), Membranes with bound oligonucleotides and peptides.
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  22. Erlich Henry A. (Oakland CA) Horn Glenn T. (Framingham MA) Bugawan Teodorica (San Leandro CA) Begovich Ann B. (El Cerrito CA), Method for HLA DP typing.
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  23. Augenlicht Leonard (Stamford CT), Method for detecting pathological conditions.
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  24. Geysen Hendrik M. (Knoxfield AUX), Method for determining mimotopes.
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  25. White Bruce A. (Farmington CT) Bancroft F. Carter (Brooklyn NY), Method for simple analysis of relative nucleic acid levels in multiple small samples by cytoplasmic dot hybridization.
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  26. Landegren Ulf (Pasadena CA) Hood Leroy (Pasadena CA), Method of detecting a nucleotide change in nucleic acids.
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  27. Fung Steven (Palo Alto CA) Woo Sam L. (Redwood City CA) Haugland Richard P. (Junction City OR) Menchen Steven M. (Hayward CA) Connell Charles R. (Redwood City CA), Method of detecting electrophoretically separated oligonucleotides.
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  28. Bahl Chander (Flemington NJ) Lang Rhonda (Lawrenceville NJ) Mendoza Leopoldo G. (Madison WI), Method of immobilizing nucleic acid on a solid surface for use in nucleic acid hybridization assays.
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  29. Gray Joe W. (Livermore CA) Pinkel Daniel (Walnut Creek CA), Method of preparing and applying single stranded DNA probes to double stranded target DNAs in situ.
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  30. Drmanac Radoje T. (Zvecanska 46 Beograd 11000) Crkvenjakov Radomir B. (Bulevar JNA 118 Beograd YUX 11000), Method of sequencing of genomes by hybridization of oligonucleotide probes.
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  31. Apple Raymond J. (San Francisco CA) Erlich Henry A. (Oakland CA) Griffith Robert L. (Belmont CA) Scharf Stephen J. (Albany CA), Methods and reagents for HLA DRbeta DNA typing.
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  32. Gray Joe W. (Livermore CA) Pinkel Daniel (Walnut Creek CA), Methods for chromosome-specific staining.
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  33. Madou Marc J. (Palo Alto CA) Otagawa Takaaki (Fremont CA), Microelectrochemical sensor and sensor array.
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  34. Ward David C. (Guilford CT) Langer Pennina R. (Monsey NY) Waldrop ; III Alexander A. (Charlottesville VA), Modified nucleotides and methods of preparing and using same.
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  35. Wang Chang-Ning J. (Chelmsford MA) Klotz Lynn C. (Cambridge MA), Nucleic acid hybridization assays.
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  36. Macevicz Stephen C. (21890 Rucker Dr. Cupertino CA 95014), Nucleic acid sequence determination by multiple mixed oligonucleotide probes.
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  37. Dattagupta Nanibhushan (New Haven CT) Albarella James P. (Elkhart IN), Photochemical nucleic acid-labeling reagent having a polyalklamine spacer.
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  38. Humphries Gillian M. K. (Los Altos CA) Parce John W. (Winston-Salem NC) McConnell Harden M. (Stanford CA), Photoresponsive redox detection and discrimination.
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  39. Frank Ronald (Wolfenbttel DEX) Heikens Wiebke (Braunschweig DEX), Process for the simultaneous synthesis of several oligonucleotides on a solid phase.
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  40. Clark Noel A. (Boulder CO) Douglas Kenneth (Boulder CO) Rothschild Kenneth J. (Newton MA), Self-assembled nanometer lithographic masks and templates and method for parallel fabrication of nanometer scale multi-d.
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  41. Southern Edwin M. (Oxford GB3) Maskos Uwe (Oxford GB3), Support-bound oligonucleotides.
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  42. Cozzette Stephen N. (Nepean CAX) Davis Graham (Plainsboro NJ) Itak Jeanne A. (North Brunswick NJ) Lauks Imants R. (Yardley PA) Mier Randall M. (Morrisville PA) Piznik Sylvia (Jackson NJ) Smit Nicolaa, Wholly microfabricated biosensors and process for the manufacture and use thereof.
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