초록 ▼

A reagent is suitable for measuring the concentration of an analyte in a hemoglobin-containing biological fluid, such as whole blood. The reagent comprises dehydrogenase enzyme that has specificity for the analyte, NAD, an NAD derivative, pyrrolo-quinoline quinone (PQQ), or a PQQ derivative, a tetra

A reagent is suitable for measuring the concentration of an analyte in a hemoglobin-containing biological fluid, such as whole blood. The reagent comprises dehydrogenase enzyme that has specificity for the analyte, NAD, an NAD derivative, pyrrolo-quinoline quinone (PQQ), or a PQQ derivative, a tetrazolium dye precursor, a diaphorase enzyme or an analog thereof, and a nitrite salt. The reagent causes dye formation that is a measure of the analyte concentration. The nitrite salt suppresses interfering dye formation caused non-enzymatically by the hemoglobin. Preferably, the reagent is used in a dry strip for measuring ketone bodies, such as beta-hydroxybutyrate.

대표청구항

[What is claimed is:] [1.]a) a dehydrogenase enzyme that has specificity for the analyte,b) pyrrolo-quinoline quinone(PQQ), or a PQQ derivativec) a tetrazolium dye precursor,d) a diaphorase enzyme or an analog thereof, ande) a nitrite salt.

[What is claimed is:] [1.]a) a dehydrogenase enzyme that has specificity for the analyte,b) pyrrolo-quinoline quinone(PQQ), or a PQQ derivativec) a tetrazolium dye precursor,d) a diaphorase enzyme or an analog thereof, ande) a nitrite salt.