초록 ▼

A culturing system and method particularly useful for producing cellular products such as viral pathogens of cells. It includes a mass transfer culture segment, stacked filter plates to adjust the medium composition, and a product removal and concentration segment. The mass transfer culture segment

A culturing system and method particularly useful for producing cellular products such as viral pathogens of cells. It includes a mass transfer culture segment, stacked filter plates to adjust the medium composition, and a product removal and concentration segment. The mass transfer culture segment utilizes changed directional flow of the medium to maximize cell growth and production of product. The stacked filter plates allow addition of sterile fresh medium and removal of growth inhibitory substances.

대표청구항 ▼

[ What is claimed is:] [1.]1. A method for separating one or more components from an input fluid, said method comprising:(a) providing a first tangential flow device comprising:(i) a first housing enclosing an interior volume;(ii) one or more filter means separating the interior volume of the first

[ What is claimed is:] [1.]1. A method for separating one or more components from an input fluid, said method comprising:(a) providing a first tangential flow device comprising:(i) a first housing enclosing an interior volume;(ii) one or more filter means separating the interior volume of the first housing into a first set of one or more chambers and a second set of one or more chambers;(iii) at least one entrance port and at least one exit port in fluid communication with the first set of one or more chambers;(iv) at least one entrance and/or exit port in fluid communication with the second set of one or more chambers;(b) providing a second tangential flow device comprising:(i) a second housing enclosing an interior volume;(ii) one or more filter means separating the interior volume of the second housing into a first set of one or more chambers and a second set of one or more chambers;(iii) at least one entrance port and at least one exit port in fluid communication with the first set of one or more chambers;(iv) at least one entrance and/or exit port in fluid communication with the second set of one or more chambers;(c) in the first tangential flow device, introducing a first fluid via a port into the first set of one or more chambers, such that a permeate comprising a first permeate component from the first fluid can traverse the one or more filter means and enter the second set of one or more chambers to provide a second fluid comprising the first permeate component;(d) removing the second fluid from the second set of one or more chambers of the first tangential flow device via an exit port; and(e) in the second tangential flow device, introducing the second fluid collected in step (d) via an entrance port into the first set of one or more chambers, such that a permeate comprising the first permeate component and/or comprising a second permeate component can traverse the one or more filter means and enter the second set of one or more chambers to provide a third fluid comprising the first and/or second permeate component.