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Method of isolating RNA 원문보기

IPC분류정보
국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판)
  • C07H-021/04
  • C12Q-001/68
  • C12P-019/34
  • B01D-015/08
출원번호 US-0445944 (1999-12-20)
국제출원번호 PCT/US98/13180 (1998-06-25)
§371/§102 date 19991220 (19991220)
국제공개번호 WO-9859076 (1998-12-30)
발명자 / 주소
  • Ekenberg Steven J.
출원인 / 주소
  • Promega Corporation
대리인 / 주소
    Michael Best & Friedrich LLPFrenchick
인용정보 피인용 횟수 : 57  인용 특허 : 4

초록 ▼

The present invention provides a method for isolating RNA from a biological material comprising RNA and contaminants, wherein: the biological material is disrupted in the presence of a chaotropic agent, the resulting lysate is diluted to precipitate out contaminants, and the precipitate is removed f

대표청구항 ▼

[ What is claimed is:] [1.]1. A method of isolating RNA from a biological material, comprising the steps ofa) providing a biological material comprising RNA and contaminants, wherein the contaminants include DNA and proteins;b) disrupting the biological material in a first container in the presence

이 특허에 인용된 특허 (4)

  1. Wolin Christopher D. (Milwaukee WI) Franciskovich Phillip P. (Brown Deer WI), M-RNA purification.
    상세보기
  2. Padhye Vikas V. (Madison WI) York Chuck (Madison WI) Burkiewicz Adam (Gdansk PLX), Nucleic acid purification on silica gel and glass mixtures.
    상세보기
  3. Gillespie David (Glenmoore PA) Cuddy Kevin K. (Chester Springs PA), Process and kit for isolating and purifying RNA from biological sources.
    상세보기
  4. Little Michael C. (Martinez CA), Process for the purification of DNA on diatomaceous earth.
    상세보기

이 특허를 인용한 특허 (57)

  1. Zielenski,Ralf; Geissler,Klaus; Walter,Thomas, Adsorption of nucleic acids to a solid phase.
    상세보기
  2. Singer, Thorsten, Apparatus and method for the more efficient isolation of nucleic acids.
    상세보기
  3. Heath, Ellen M.; Shuman, Ruth M., Apparatuses and methods for isolating nucleic acid.
    상세보기
  4. Bavykin, Sergei G.; Akowski, James P.; Zakhariev, Vladimir M.; Mirzabekov, Andrei, Column device for isolation and labeling of nucleic acids.
    상세보기
  5. Fernando, Rohan M.; Ryan, Wayne L.; Hunsley, Brad, Compositions and methods for stabilizing circulating tumor cells.
    상세보기
  6. Bair, Robert Jackson; Benedict, Kristen Campbell; Kivens, Wendy J.; Kwiatkowski, Jr., Robert W.; Paulsen, Kim; Strom, Daniel A.; Wages, John M., Compositions and methods for using a solid support to purify DNA.
    상세보기
  7. Bair, Jr., Robert Jackson; Heath, Ellen M.; Meehan, Heather; Paulsen, Kim Elayne; Wages, Jr., John M., Compositions and methods for using a solid support to purify RNA.
    상세보기
  8. Bair, Jr.,Robert Jackson; Heath,Ellen M.; Meehan,Heather; Paulsen,Kim Elayne; Wages, Jr.,John M., Compositions and methods for using a solid support to purify RNA.
    상세보기
  9. Helftenbein,Elke, Container for nucleic acid analysis.
    상세보기
  10. Pasloske, Brittan; Hoang, Quoc, Crude biological derivatives competent for nucleic acid detection.
    상세보기
  11. Pasloske, Brittan; Hoang, Quoc, Crude biological derivatives competent for nucleic acid detection.
    상세보기
  12. Pasloske, Brittan; Hoang, Quoc, Crude biological derivatives competent for nucleic acid detection.
    상세보기
  13. Reed, Michael W.; Nanassy, Oliver Z., Device and method for extraction and analysis of nucleic acids from biological samples.
    상세보기
  14. Reed, Michael W.; Nanassy, Oliver Z., Device and method for extraction and analysis of nucleic acids from biological samples.
    상세보기
  15. Reed, Michael W.; Nanassy, Oliver Z.; Haydock, Paul V.; Sharma, Nigel Rudra; Bardell, Ronald L.; Hargrave, Perry, Device and method for extraction and analysis of nucleic acids from biological samples.
    상세보기
  16. Fernando, M. Rohan, Devices and compositions for preservation of cell-free nucleic acids.
    상세보기
  17. Reed, Michael W.; Nanassy, Oliver Z.; Haydock, Paul V.; Sharma, Nigel Rudra; Bardell, Ronald L.; Hargrave, Perry, Devices and processes for nucleic acid extraction.
    상세보기
  18. Fare, Thomas L.; Kessler, Deborah A.; Kilian, Kristopher A., Extraction and diagnostic fluid devices, systems and methods of use.
    상세보기
  19. Richardson,Derrick A.; Pai,Derek S.; Monteon,Jorge, Filtration column devices and methods of filtering therewith.
    상세보기
  20. Reed, Michael W.; Gestwick, Daniel P., Gravity flow fluidic device for nucleic acid extraction.
    상세보기
  21. Ryan, Wayne L.; Grunkemeyer, James A, Inactivated virus compositions and methods of preparing such compositions.
    상세보기
  22. Deggerdal, Arne Helge; Larsen, Frank, Isolation of nucleic acid.
    상세보기
  23. Deggerdal, Arne; Larsen, Frank, Isolation of nucleic acid.
    상세보기
  24. Pai, Derek S.; Kunitake, Steven T.; Richardson, Derrick; Monteon, Jorge, Low volume filtration column devices and methods of filtering therewith.
    상세보기
  25. Smith, Thomas L.; Pai, Derek S.; Kunitake, Steven T.; Richardson, Derrick; Monteon, Jorge, Low volume filtration column devices and methods of filtering therewith.
    상세보기
  26. Augello, Frank A.; Rainen, Lynne; Walenciak, Matthew; Oelmuller, Uwe; Wyrich, Ralf; Bastian, Helge, Method and device for collecting and stabilizing a biological sample.
    상세보기
  27. Hillebrand, Timo, Method and formulation for the extraction of nucleic acids from any complex starting materials.
    상세보기
  28. Hucklenbroich,Joerg; Mueller,Markus, Method for coarse purification of cell digests from microorganisms.
    상세보기
  29. Chen, Yi-Chang; Tsai, Jane S-C, Method for isolating nucleic acids and composition used therefor.
    상세보기
  30. Bosnes, Marie, Method for isolating nucleic acids and protein from a single sample.
    상세보기
  31. Inomata, Hiroko; Iwata, Rie, Method for selectively separating and purifying RNA and method for separating and purifying nucleic acid.
    상세보기
  32. Inomata, Hiroko; Mori, Tomoko, Method for separating and purifying RNA.
    상세보기
  33. Haj-Ahmad, Yousef, Methods and columns for nucleic acid purification.
    상세보기
  34. Haj-Ahmad, Yousef, Methods and columns for nucleic acid purification.
    상세보기
  35. Conrad, Richard, Methods and compositions for isolating small RNA molecules.
    상세보기
  36. Conrad, Richard, Methods and compositions for isolating small RNA molecules.
    상세보기
  37. Conrad, Richard C., Methods and compositions for isolating small RNA molecules.
    상세보기
  38. Fernando, M. Rohan, Methods for preservation of cell-free nucleic acids.
    상세보기
  39. Bitner, Rex M., Methods of optimal purification of nucleic acids and kit for use in performing such methods.
    상세보기
  40. Bitner, Rex M., Methods of purifying a nucleic acid and formulation and kit for use in performing such methods.
    상세보기
  41. Bitner, Rex M., Methods of purifying a nucleic acid and formulation and kit for use in performing such methods.
    상세보기
  42. Bitner, Rex M.; Mandrekar, Michelle N.; Brisco, Paula R., Nucleic acid purification with a binding matrix.
    상세보기
  43. Bitner, Rex M.; Mandreker, Michelle M.; Brisco, Paula R., Nucleic acid purification with a binding matrix.
    상세보기
  44. Fernando, M. Rohan, Preservation of cell-free RNA in blood samples.
    상세보기
  45. Fernando, M. Rohan, Preservation of cell-free RNA in blood samples.
    상세보기
  46. Fernando, M. Rohan, Preservation of cell-free nucleic acids.
    상세보기
  47. Holländer, Vera, Process for parallel isolation and/or purification of RNA and DNA.
    상세보기
  48. Kore, Anilkumar R.; Shanmugasundaram, Muthian, Proteinase K inhibitors, methods and compositions therefor.
    상세보기
  49. Kore, Anilkumar R.; Shanmugasundaram, Muthian, Proteinase K inhibitors, methods and compositions therefor.
    상세보기
  50. Paul, John H.; John, David E., RNA-based system and method to differentiate seafood.
    상세보기
  51. Belly,Robert; Toner,Jacqueline; Backus,John, Rapid extraction of RNA from cells and tissues.
    상세보기
  52. Baker, Tony, Removal of molecular assay interferences.
    상세보기
  53. Fekete, Richard A.; Nguyen, Annalee, Sample preparation for in situ nucleic acid analysis, methods and compositions therefor.
    상세보기
  54. Fekete, Richard; Nguyen, Annalee, Sample preparation for in situ nucleic acid analysis, methods and compositions therefor.
    상세보기
  55. Fekete, Richard; Nguyen, Annalee, Sample preparation for in situ nucleic acid analysis, methods and compositions therefor.
    상세보기
  56. Bavykin, Sergei, Universal nucleic acids sample preparation method for cells, spores and their mixture.
    상세보기
  57. Helftenbein, Elke, Vessel for blood sampling.
    상세보기

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