$\require{mediawiki-texvc}$

연합인증

연합인증 가입 기관의 연구자들은 소속기관의 인증정보(ID와 암호)를 이용해 다른 대학, 연구기관, 서비스 공급자의 다양한 온라인 자원과 연구 데이터를 이용할 수 있습니다.

이는 여행자가 자국에서 발행 받은 여권으로 세계 각국을 자유롭게 여행할 수 있는 것과 같습니다.

연합인증으로 이용이 가능한 서비스는 NTIS, DataON, Edison, Kafe, Webinar 등이 있습니다.

한번의 인증절차만으로 연합인증 가입 서비스에 추가 로그인 없이 이용이 가능합니다.

다만, 연합인증을 위해서는 최초 1회만 인증 절차가 필요합니다. (회원이 아닐 경우 회원 가입이 필요합니다.)

연합인증 절차는 다음과 같습니다.

최초이용시에는
ScienceON에 로그인 → 연합인증 서비스 접속 → 로그인 (본인 확인 또는 회원가입) → 서비스 이용

그 이후에는
ScienceON 로그인 → 연합인증 서비스 접속 → 서비스 이용

연합인증을 활용하시면 KISTI가 제공하는 다양한 서비스를 편리하게 이용하실 수 있습니다.

PCR method for nucleic acid quantification utilizing second or third order rate constants 원문보기

IPC분류정보
국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판)
  • C12Q-001/68
  • C12P-019/34
출원번호 US-0635344 (2000-08-09)
발명자 / 주소
  • Wittwer Carl T.
  • Ririe Kirk M.
  • Rasmussen Randy P.
출원인 / 주소
  • University of Utah Research Foundation
대리인 / 주소
    Barnes & Thornburg
인용정보 피인용 횟수 : 68  인용 특허 : 50

초록 ▼

The present invention is directed to a method of determining the concentration of a nucleic acid product that had been amplified through polymerase chain reaction (PCR). More particularly, the present invention relates to a method wherein a rate constant is determined for a known concentration of am

대표청구항 ▼

[ We claim:] [1.]1. A method of determining a concentration of an amplified product in a selected polymerase chain reaction mixture comprising(a) determining a second order rate constant for the amplified product at a selected temperature and reaction conditions by monitoring rate of hybridization o

이 특허에 인용된 특허 (50)

  1. Stavrianopoulos Jannis (New York NY) Rabbani Elazar (New York NY) Abrams Samuel B. (New York NY) Wetmur James G. (Scardsdale NY), Analyte detection by means of energy transfer.
    상세보기
  2. Zaun Peter (Libertyville IL) Bouma Stanley R. (Grayslake IL) Gordon Julian (Lake Bluff IL) Kotlarik John J. (Vernon Hills IL), Apparatus and method for amplifying and detecting target nucleic acids.
    상세보기
  3. Johnson Larry J. (San Jose CA) Widunas Joseph T. (Berkeley CA), Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooli.
    상세보기
  4. Mullis Kary B. (La Jolla CA) Johnson Larry (San Jose CA) Leath Richard A. (Berkley CA) Wennberg Timothy J. (Mariposa CA) Mezei Louis M. (Madison WI) Widunas Joseph T. (Freemont CA), Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooli.
    상세보기
  5. Oka Hisao (Nagoya MD JPX) Ito Yoichiro (Bethesda MD), Apparatus for countercurrent chromatography separations.
    상세보기
  6. Hosoi Shigeru (Hamamatsu JPX) Fukami Tadashi (Hamamatsu JPX) Tsuji Akihiko (Hamamatsu JPX), Apparatus for detecting denaturation of nucleic acid.
    상세보기
  7. Rusnak Miro (LaVerne CA) Shively John E. (Arcadia CA) Calaycay Jimmy R. (Arcadia CA), Apparatus for the sequential performance of chemical processes.
    상세보기
  8. Hallsby G. Anders (Midland MI), Automatic air temperature cycler and method of use in polymerose chain reaction.
    상세보기
  9. Suzuki Yoshiyuki (Tokyo JPX) Kato Takahiko (Chigasaki JPX), Automatic cycling reaction apparatus and automatic analyzing apparatus using the same.
    상세보기
  10. Zare Richard N. (Stanford CA) Tsuda Takao (Palo Alto CA), Capillary electrophoresis injection device and method.
    상세보기
  11. Allington Robert W. (Lincoln NE) Allington John R. (Lincoln NE), Capillary electrophoresis technique.
    상세보기
  12. Tehrani Yoossef (Lincoln NE), Capillary electrophoresis technique.
    상세보기
  13. Andresen Brian D. (Pleasanton CA) Saxena Vinit (Pinole CA), Chromatography column-electrophoresis system.
    상세보기
  14. Anderwald Cecil E. (3077 Windmill La. Dallas TX 75234), Container means preventing accidental use by children.
    상세보기
  15. Dutton Edmund L. (Marietta OH) Selfridge Glenn D. (Marietta OH) Tipton Russell C. (Williamstown WV), Controlled atmosphere enclosure.
    상세보기
  16. Malick Franklin S. (518 Greenleaf Dr. Monroeville PA 15146), Cooking appliance.
    상세보기
  17. Haugland Richard P. (Eugene OR) Yue Stephen T. (Eugene OR) Millard Paul J. (Eugene OR) Roth Bruce L. (Corvallis OR), Cyclic-substituted unsymmetrical cyanine dyes.
    상세보기
  18. Tyagi Sanjay ; Kramer Fred R. ; Lizardi Paul M.,MXX, Detectably labeled dual conformation oligonucleotide probes, assays and kits.
    상세보기
  19. Stapleton Marilyn J. (Durham NC) Thorpe Villette S. (Goldsboro NC) Jewett Warren R. (Cary NC), Devices for containing biological specimens for thermal processing.
    상세보기
  20. Sutton Richard C. (Rochester NY) Ponticello Ignazio S. (Pittsford NY) Cummins Thomas J. (Rochester NY) Zander Dennis R. (Penfield NY) Donish William H. (Rochester NY), Element and method for nucleic acid amplification and detection using adhered probes.
    상세보기
  21. Varano Antonio (Philadelphia PA) Tobias Reginald (Watertown MA), Gas chromatograph for continuous operation with infrared spectrometer.
    상세보기
  22. Sisti Giorgio (Melzo ITX) Riva Ermete (Merate ITX) Sala Learco (Monza ITX), Gas chromatographic chamber.
    상세보기
  23. Howe Robert L. (San Ramon CA), Gas chromatographic oven using symmetrical flow of preheated - premixed ambient air.
    상세보기
  24. Ray Curtis (Benicia CA), Heating and cooling apparatus for chromatography column.
    상세보기
  25. Gelfand David H. (Oakland CA) Holland Pamela M. (Oakland CA) Saiki Randall K. (Richmond CA) Watson Robert M. (Berkeley CA), Homogeneous assay system using the nuclease activity of a nucleic acid polymerase.
    상세보기
  26. Sutherland John W. H. (Rochester NY) Patterson David R. (Rochester NY), Homogeneous method for assay of double-stranded nucleic acids using fluorescent dyes and kit useful therein.
    상세보기
  27. Heller Michael J. (Encinitas CA), Hybridization of polynucleotides conjugated with chromophores and fluorophores to generate donor-to donor energy transfe.
    상세보기
  28. Atwood John G. (West Redding CT) Haff Lawrence A. (Wilton CT), In situ PCR amplification system.
    상세보기
  29. Hall Arlie (4204 Rolling Hills Dr. Lexington KY 40511), Insulating cell element and structures composed thereof.
    상세보기
  30. Zare Richard N. (Stanford CA) Gassmann Ernst (Basel CHX), Laser-excitation fluorescence detection electrokinetic separation.
    상세보기
  31. Grosz Ron (Wilmington DE) Jensen Mark A. (West Chester PA), Method for amplification of targeted segments of nucleic acid using nested polymerase chain reaction.
    상세보기
  32. Bouma Stanley R. (Grayslake IL) Khalil Omar S. (Libertyville IL) Pabich Edward K. (Chicago IL), Method for detection of nucleic acid using total internal reflectance.
    상세보기
  33. Wang Chang-Ning J. (Chelmsford MA) Wu Kai-Wuan (Lowell MA), Method for reducing non-specific priming in DNA amplification.
    상세보기
  34. Hjerten Stellan (Upsala SEX) Srichaiyo Tasanee (Upsala SEX), Multi-point detection method for electrophoresis and chromatography in capillaries.
    상세보기
  35. Butcher Eugene C. (Portola Valley CA) Picker Louis J. (Dallas TX), Neutrophil LECAM-1 as indicator of neutrophil activation.
    상세보기
  36. Afeyan Noubar B. (Brookline MA) Regnier Fred E. (West Lafayette IN), On-line product identification in a chromatography effluent by subtraction.
    상세보기
  37. Mullis Kary B. (Kensington CA), Process for amplifying nucleic acid sequences.
    상세보기
  38. Mullis Kary B. (La Jolla CA) Erlich Henry A. (Oakland CA) Gelfand David H. (Oakland CA) Horn Glenn (Emeryville CA) Saiki Randall K. (Richmond CA), Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme.
    상세보기
  39. Mullis Kary B. (Kensington CA) Erlich Henry A. (Oakland CA) Arnheim Norman (Woodland Hills CA) Horn Glenn T. (Emeryville CA) Saiki Randall K. (Richmond CA) Scharf Stephen J. (Berkeley CA), Process for amplifying, detecting, and/or-cloning nucleic acid sequences.
    상세보기
  40. Gelfand David H. (Oakland CA) Stoffel Susanne (El Cerrito CA) Lawyer Frances C. (Oakland CA) Saiki Randall K. (Richmond CA), Purified thermostable enzyme.
    상세보기
  41. Wittwer Carl T. (Salt Lake City UT) Hillyard David R. (Salt Lake City UT) Ririe Kirk M. (Idaho Falls ID), Rapid thermal cycling device.
    상세보기
  42. Coakley Joseph (Woodland CA) Godsey James (Folsom CA) Sherman David (Sacramento CA), Sealable vessel for containing and processing analytical samples.
    상세보기
  43. Pace Salvatore J. (Wilmington DE), Silicon semiconductor wafer for analyzing micronic biological samples.
    상세보기
  44. Leaseburge Emory J. (Lewisburg WV) Thomas Thomas J. (Lewisburg WV), Supercritical fluid chromatograph with pneumatically controlled pump.
    상세보기
  45. Chien Ring-Ling (San Jose CA) Burgi Dean S. (Palo Alto CA), System and method for improving sample concentration in capillary electrophoresis.
    상세보기
  46. Coy Richard A. (Grass Lake MI) Waycaster Roy A. (Tecumseh MI), Temperature control apparatus.
    상세보기
  47. Columbus Richard L. (Rochester NY) Helfer Jeffrey L. (Webster NY) Porte Johannes J. (Webster NY) Wellman Jeffrey A. (Rochester NY), Temperature cycling cuvette.
    상세보기
  48. Lane Michael J. ; Benight Albert S. ; Faldasz Brian D., Thermodynamics, design, and use of nucleic acid sequences.
    상세보기
  49. Jorgenson James W. (Chapel Hill NC) Bushey Michelle M. (San Antonio TX), Two-dimensional high-performance liquid chromatography/capillary electrophoresis.
    상세보기
  50. Jorgenson James W. (Chapel Hill NC) Bushey Michelle M. (San Antonio TX), Two-dimensional high-performance liquid chromatography/capillary electrophoresis.
    상세보기

이 특허를 인용한 특허 (68)

  1. Katz,David Aaron; Gentile Davey,Maria C., Amplification based polymorphism detection.
    상세보기
  2. Riley, Timothy A.; Brown, Bob D.; Arnold, Lyle J., Antisense oligonucleotides with increased RNase sensitivity.
    상세보기
  3. McMillan, William A.; Christel, Lee A.; Borkholder, David A.; Young, Steven J., Apparatus for analysis of a nucleic acid amplification reaction.
    상세보기
  4. McMillan, William A.; Christel, Lee A.; Borkholder, David A.; Young, Steven J., Apparatus for quantitative analysis of a nucleic acid amplification reaction.
    상세보기
  5. Maltezos, George; Johnston, Matthew; Goodwin, David; Scherer, Axel; Walker, Christopher I., Apparatus for thermal cycling.
    상세보기
  6. Ochranek, Brian L.; Arnquist, David C.; Oonuma, Takehiko; Tahara, Hirotoshi; Sato, Naoto; Smith, Bradley P., Automated diagnostic analyzers having rear accessible track systems and related methods.
    상세보기
  7. Ochranek, Brian L.; Arnquist, David C.; Oonuma, Takehiko; Tahara, Hirotoshi; Sato, Naoto, Automated diagnostic analyzers having vertically arranged carousels and related methods.
    상세보기
  8. Ochranek, Brian L.; Arnquist, David C.; Oonuma, Takehiko; Tahara, Hirotoshi; Sato, Naoto, Automated diagnostic analyzers having vertically arranged carousels and related methods.
    상세보기
  9. Linnen, Jeffrey M.; Kolk, Daniel P.; Dockter, Janel M.; Getman, Damon K.; Yoshimura, Tadashi; Ho-Sing-Loy, Martha K.; Pollner, Reinhold B.; Stringfellow, Leslie A., Compositions and methods for detecting hepatitis B virus.
    상세보기
  10. Linnen, Jeffrey M.; Kolk, Daniel P.; Dockter, Janel M.; Getman, Damon K.; Yoshimura, Tadashi; Ho-Sing-Loy, Martha K.; Pollner, Reinhold B.; Stringfellow, Leslie A., Compositions and methods for detecting hepatitis B virus.
    상세보기
  11. Linnen, Jeffrey M.; Kolk, Daniel P.; Dockter, Janel M.; Getman, Damon K.; Yoshimura, Tadashi; Ho-Sing-Loy, Martha K.; Pollner, Reinhold B.; Stringfellow, Leslie A., Compositions and methods for detecting hepatitis B virus.
    상세보기
  12. Siegel, Donald L., Compositions, methods and kits for detection of an antigen on a cell and in a biological mixture.
    상세보기
  13. Lair, Gary D.; Nguyen, Thanh N.; Li, Haitao; Li, Florence; Knight, Byron J.; Heinz, Robert E.; Macioszek, Jerzy A.; Davis, Christopher B.; Scalese, Robert F., Continuous process for performing multiple nucleic acid amplification assays.
    상세보기
  14. Ochranek, Brian L.; Arnquist, David C., Diagnostic analyzers with pretreatment carousels and related methods.
    상세보기
  15. Breidenthal, Scott S.; Fan, Sara H.; Lee, Richard S.; Nelson, Norman C.; Scott, Matthew J.; Taylor, Jason A., Gravity-assisted mixing methods.
    상세보기
  16. Breidenthal, Scott S.; Lee, Richard S.; Nelson, Norman C.; Scott, Matthew J.; Taylor, Jason A.; Fan, Sara H., Gravity-assisted mixing methods.
    상세보기
  17. Boles, Deborah; Hasson, Kenton C.; Kanderian, Sami, High-resolution melting analysis.
    상세보기
  18. Boles, Deborah; Hasson, Kenton C.; Kanderian, Sami, High-resolution melting analysis.
    상세보기
  19. Breidenthal, Scott S.; Fan, Sara H.; Lee, Richard S.; Nelson, Norman C.; Poirier, Michael S.; Scott, Matthew J.; Taylor, Jason A., Instruments and methods for exposing a receptacle to multiple thermal zones.
    상세보기
  20. Breidenthal, Scott S.; Fan, Sara H.; Lee, Richard S.; Nelson, Norman C.; Scott, Matthew J.; Taylor, Jason A., Instruments and methods for mixing the contents of a detection chamber.
    상세보기
  21. Breidenthal, Scott S.; Fan, Sara H.; Lee, Richard S.; Nelson, Norman C.; Scott, Matthew J.; Taylor, Jason A., Instruments and methods for mixing the contents of a detection chamber.
    상세보기
  22. Breidenthal, Scott S.; Fan, Sara H.; Lee, Richard S.; Nelson, Norman C.; Scott, Matthew J.; Taylor, Jason A., Instruments and methods for mixing the contents of a detection chamber.
    상세보기
  23. Fan, Sara H.; Lee, Richard S., Instruments for mixing the contents of a detection chamber.
    상세보기
  24. Arciniegas, German; Ceremony, Jeff; Chu, Daniel Y.; Ragsdale, Charles W., Localized temperature control for spatial arrays of reaction media.
    상세보기
  25. Arciniegas, German; Ceremony, Jeff; Chu, Daniel Y.; Ragsdale, Charles W., Localized temperature control for spatial arrays of reaction media.
    상세보기
  26. Ceremony, Jeff; Chu, Daniel Y., Localized temperature control for spatial arrays of reaction media.
    상세보기
  27. Cao, Weidong, Method and system for temperature correction in thermal melt analysis.
    상세보기
  28. Macioszek, Jerzy A.; Davis, Christopher B.; Lair, Gary D.; Nguyen, Thanh N.; Li, Haitao; Li, Florence F.; Knight, Byron J.; Scalese, Robert F.; Heinz, Robert E., Method for continuous mode processing of the contents of multiple reaction receptacles in a real-time amplification assay.
    상세보기
  29. Light, II, James P.; Carrick, James M., Method for determining the amount of an analyte in a sample.
    상세보기
  30. Sagner,Gregor; Tabiti,Karim; Gutekunst,Martin; Soong,Richie, Method for determining the efficiency of nucleic acid amplifications.
    상세보기
  31. Sagner,Gregor; Tabiti,Karim; Gutekunst,Martin; Soong,Richie, Method for determining the efficiency of nucleic acid amplifications.
    상세보기
  32. McMillan, William A.; Christel, Lee A.; Borkholder, David A.; Young, Steven J., Method for quantitative analysis of a nucleic acid amplification reaction.
    상세보기
  33. Sagner, Gregor; Tabiti, Karim; Gutekunst, Martin; Soong, Richie, Method for real-time quantification of nucleic acids.
    상세보기
  34. Sagner, Gregor; Tabiti, Karim; Gutekunst, Martin; Soong, Richie, Method for the efficiency-corrected real-time quantification of nucleic acids.
    상세보기
  35. Sagner, Gregor; Tabiti, Karim; Gutekunst, Martin; Soong, Richie, Method for the efficiency-corrected real-time quantification of nucleic acids.
    상세보기
  36. Breidenthal, Scott S.; Fan, Sara H.; Lee, Richard S.; Nelson, Norman C.; Scott, Matthew J.; Taylor, Jason A., Methods and instruments for processing a sample in a multi-chambered receptacle.
    상세보기
  37. Pont-Kingdon, Genevieve; Lyon, Elaine, Methods for identifying chromosomal aneuploidy.
    상세보기
  38. Nelson, Norman C.; Roma, Gianluca, Methods for manipulating liquid substances in multi-chambered receptacles.
    상세보기
  39. Nelson, Norman C.; Roma, Gianluca, Methods for manipulating liquid substances in multi-chambered receptacles.
    상세보기
  40. McMillan, William A.; Christel, Lee A.; Borkholder, David A.; Young, Steven J., Methods for quantitative analysis of nucleic acid amplification reaction.
    상세보기
  41. Breidenthal, Scott S.; Fan, Sara H.; Lee, Richard S.; Nelson, Norman C.; Scott, Matthew J.; Taylor, Jason A., Methods of concentrating an analyte.
    상세보기
  42. Sakai, Stanley H.; McMillan, William, Methods, apparatus, and computer programs for verifying the integrity of a probe.
    상세보기
  43. Sakai, Stanley H.; McMillan, William, Methods, apparatus, and computer programs for verifying the integrity of a probe.
    상세보기
  44. Sakai,Stanley H.; McMillan,William, Methods, apparatus, and computer programs for verifying the integrity of a probe.
    상세보기
  45. Coursey, Johnathan S., Microfluidic devices with integrated resistive heater electrodes including systems and methods for controlling and measuring the temperatures of such heater electrodes.
    상세보기
  46. Coursey, Johnathan S., Microfluidic devices with integrated resistive heater electrodes including systems and methods for controlling and measuring the temperatures of such heater electrodes.
    상세보기
  47. Lerner, Jeffrey, Multi-stage, regression-based PCR analysis system.
    상세보기
  48. Lerner, Jeffrey, Multi-stage, regression-based PCR analysis system.
    상세보기
  49. Lerner, Jeffrey, Multi-stage, regression-based PCR analysis system.
    상세보기
  50. Lewis, Martin K.; Kephart, Daniel; Rhodes, Richard Byron; Shultz, John William; Leippe, Donna; Mandrekar, Michelle; Andrews, Christine Ann; Hartnett, James Robert; Gu, Trent; Wood, Keith V.; Welch, R, Multiplex method for nucleic acid detection.
    상세보기
  51. Brown, Bob D.; Riley, Timothy A., Oligonucleotide probes and primers comprising universal bases for diagnostic purposes.
    상세보기
  52. Fawcett, Adrian; Tracy, David; Simmons, Kevin Daley, Optical system for multiple reactions.
    상세보기
  53. Hasson, Kenton C.; Dale, Gregory A.; Inoue, Hiroshi, Real-time PCR in micro-channels.
    상세보기
  54. Hasson, Kenton C.; Dale, Gregory A.; Inoue, Hiroshi, Real-time PCR in micro-channels.
    상세보기
  55. Hasson, Kenton C.; Dale, Gregory A.; Inoue, Hiroshi, Real-time PCR in micro-channels.
    상세보기
  56. Knight, Byron J.; Li, Haitao, System for performing multi-formatted assays.
    상세보기
  57. Knight, Byron J., System for processing contents of a receptacle to detect an optical signal emitted by the contents.
    상세보기
  58. Hayes, Matthew J.; Knight, Byron J.; Li, Haitao; Scalese, Robert F., Systems and methods for detecting multiple optical signals.
    상세보기
  59. Hayes, Matthew J.; Knight, Byron J.; Li, Haitao; Scalese, Robert F., Systems and methods for detecting multiple optical signals.
    상세보기
  60. Bartkowiak, Miroslaw; Moore, Richard L., Systems and methods for determining an amount of starting reagent using the polymerase chain reaction.
    상세보기
  61. Whitman, Douglas F.; Collins, Charles J., Systems and methods for multiplex analysis of PCR in real time.
    상세보기
  62. Whitman, Douglas F.; Collins, Charles J., Systems and methods for multiplex analysis of PCR in real time.
    상세보기
  63. Whitman, Douglas F.; Collins, Charles J., Systems and methods for multiplex analysis of PCR in real time.
    상세보기
  64. Whitman, Douglas F.; Collins, Charles J., Systems and methods for multiplex analysis of PCR in real time.
    상세보기
  65. Whitman, Douglas F.; Collins, Charles J., Systems and methods for multiplex analysis of PCR in real time.
    상세보기
  66. Maltezos, George; Johnston, Matthew; Goodwin, David; Scherer, Axel; Walker, Christopher I., Thermal cycling apparatus.
    상세보기
  67. Maltezos, George; Johnston, Matthew; Goodwin, David; Scherer, Axel; Walker, Christopher I., Thermal cycling method.
    상세보기
  68. Maltezos, George; Johnston, Matthew; Goodwin, David; Scherer, Axel; Walker, Christopher I., Thermal cycling system.
    상세보기

관련 콘텐츠

섹션별 컨텐츠 바로가기

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

AI-Helper 아이콘
AI-Helper
안녕하세요, AI-Helper입니다. 좌측 "선택된 텍스트"에서 텍스트를 선택하여 요약, 번역, 용어설명을 실행하세요.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.

선택된 텍스트

맨위로