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Sample processing to release nucleic acids for direct detection 원문보기

IPC분류정보
국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판)
  • C12Q-001/68
  • C12P-019/34
  • C12N-001/00
  • C12N-001/06
출원번호 US-0385624 (1999-08-26)
발명자 / 주소
  • Dattagupta Nanibhushan
  • Sridhar C. Nagaraja
  • Wu Whei-Kuo
출원인 / 주소
  • Applied Gene Technologies, Inc.
대리인 / 주소
    Morrison & Foerster LLP
인용정보 피인용 횟수 : 32  인용 특허 : 11

초록 ▼

The present invention describes compositions and methods for releasing nucleic acids from cells in a form that is suitable for labeling/capture, amplification, or detection in a single reagent addition step. The compositions include a lipid, membrane fluidizing compound, enzyme for degrading cell st

대표청구항 ▼

[ We claim:] [1.]1. A composition for releasing nucleic acid from a sample in a form suitable for directly detecting the nucleic acid, sad composition comprising: an aqueous solution comprising one or more lipids selected fom the gout consisting of 3-(2-aminoprpoyl 1,3-dihexadecyloxypropyl) hexadecy

이 특허에 인용된 특허 (11)

  1. Dattagupta Nanibhushan (West Haven CT) Rae Peter M. M. (Hamden CT) Rabin Daniel U. (Branford CT) Huguenel Edward D. (Guilford CT), Assay for nucleic acid sequences in an unpurified sample.
    상세보기
  2. Western Linda M. ; Rose Samuel J. ; Ullman Edwin F., Detection of nucleic acids by target-catalyzed product formation.
    상세보기
  3. Dattagupta Nanibhushan (New Haven CT) Crothers Donald M. (Northford CT), Fast photochemical method of labelling nucleic acids for detection purposes in hybridization assays.
    상세보기
  4. Clark Kathleen A. ; Kacian Daniel L., Methods for extracting nucleic acids from a wide range of organisms by nonlytic permeabilization.
    상세보기
  5. Cimino George D. (1435 Liberty St. El Cerrito CA 94530) Hearst John E. (101 Southampton Ave. Berkeley CA 94707) Isaacs Stephen T. (79 Loma Vista Dr. Orinda CA 94563) Metchette Kenneth C. (2041 Franci, Methods for rendering amplified nucleic acid subsequently unamplifiable.
    상세보기
  6. Davey Cheryl (Toronto CAX) Malek Lawrence T. (Brampton CAX), Nucleic acid amplification process.
    상세보기
  7. Davey Cheryl (Toronto CAX) Malek Lawrence T. (Brampton CAX), Nucleic acid amplification process.
    상세보기
  8. Dattagupta Nanibhushan (New Haven CT) Albarella James P. (Elkhart IN), Photochemical nucleic acid-labeling reagent having a polyalklamine spacer.
    상세보기
  9. Mullis Kary B. (Kensington CA), Process for amplifying nucleic acid sequences.
    상세보기
  10. Mullis Kary B. (Kensington CA) Erlich Henry A. (Oakland CA) Arnheim Norman (Woodland Hills CA) Horn Glenn T. (Emeryville CA) Saiki Randall K. (Richmond CA) Scharf Stephen J. (Berkeley CA), Process for amplifying, detecting, and/or-cloning nucleic acid sequences.
    상세보기
  11. Sheldon ; III Edward L. (Oakland CA) Levenson Corey H. (Oakland CA) Mullis Kary B. (Oakland CA) Rapoport Henry (Berkeley CA), Process for labeling nucleic acids using psoralen derivatives.
    상세보기

이 특허를 인용한 특허 (32)

  1. Lawrence, David S.; Pestell, Richard G.; Albanese, Christopher, Caged ligands and uses thereof.
    상세보기
  2. Birnboim, Chaim H.; Jackson, Adele; Iwasiow, Rafal; Chartier, Joanne; Lem, Paul, Compositions and method for storage of nucleic acid from bodily fluids.
    상세보기
  3. Birnboim, H. Chaim; Jackson, Adele; Iwasiow, Rafal; Chartier, Joanne; Lem, Paul, Compositions and method for storage of nucleic acid from bodily fluids.
    상세보기
  4. Gundling, Gerard J., Compositions and methods for nucleic acid extraction.
    상세보기
  5. Gundling, Gerard J., Compositions and methods for nucleic acid extraction.
    상세보기
  6. Birnboim, H. Chaim, Compositions and methods for obtaining nucleic acids from sputum.
    상세보기
  7. Birnboim,H. Chaim, Compositions and methods for obtaining nucleic acids from sputum.
    상세보기
  8. Greenfield, Lawrence; Montesclaros, Luz, Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases.
    상세보기
  9. Greenfield,I. Lawrence, Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases.
    상세보기
  10. Greenfield,Lawrence; Montesclaros,Luz, Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases.
    상세보기
  11. Muir, Rod; Kirkland, Derek; Curry, Ian; Sunstrum, Roy; Lem, Paul; Birnboim, H. Chaim, Container system for releasably storing a substance.
    상세보기
  12. Muir, Rod; Kirkland, Derek; Curry, Ian; Sunstrum, Roy; Lem, Paul; Birnboim, H. Chaim, Container system for releasably storing a substance.
    상세보기
  13. Hsieh, Hsing-Pang; Chang, Jang-Yang; Kuo, Ching-Chuan; Chao, Yu-Sheng, Coumarin compounds and their use for treating cancer.
    상세보기
  14. Richards, William L.; Ashby, Austin; Ketterer, Matthew; Marshall, Kevin; Harrison, Josh; Mette, Matthew; Richards, Paul; Showalter, Wayne; Cote, Jasmin; Halbert, Phillip C.; Bourgeois, Solene; Metzger, Steven W.; Hance, Ken; Mensack, Meghan; Michel, Carlos; Allers, Elke; Gamage, Dulini; Prisbrey, Landon; Gusyatin, Oleg; Shamsheyeva, Alena; Turng, Ben; Ghusson, Andrew; Reinhardt, Kurt, Instrument and system for rapid microogranism identification and antimicrobial agent susceptibility testing.
    상세보기
  15. Richards, William L.; Ashby, Austin; Ketterer, Matthew; Marshall, Kevin; Harrison, Josh; Mette, Matthew; Richards, Paul; Showalter, Wayne; Cote, Jasmin; Halbert, Phillip C.; Bourgeois, Solene; Metzger, Steven W.; Hance, Ken; Mensack, Meghan; Michel, Carlos; Allers, Elke; Gamage, Dulini; Prisbrey, Landon; Gusyatin, Oleg; Shamsheyeva, Alena; Turng, Ben; Ghusson, Andrew; Reinhardt, Kurt, Instrument and system for rapid microorganism identification and antimicrobial agent susceptibility testing.
    상세보기
  16. Okada, Kazushi; Ibuki, Tadayuki; Kim, Donghyun; Fujisawa, Tadashi, Liposome compositions.
    상세보기
  17. Okada, Kazushi; Ibuki, Tadayuki; Kim, Donghyun; Fujisawa, Tadashi, Liposome compositions.
    상세보기
  18. Prisbrey, Landon; Blackmore, Elise; Hance, Kenneth R.; Metzger, Steven W.; Marshall, Kevin, Membrane-assisted purification.
    상세보기
  19. Korfhage, Christian; Löffert, Dirk, Method for activating a nucleic acid for a polymerase reaction.
    상세보기
  20. Bornarth,Carole; Wisniewski,Michele; Hosono,Seiyu; Raghunathan,Arumugham; Lasken,Roger S., Method for nucleic acid amplification that results in low amplification bias.
    상세보기
  21. Dattagupta, Nanibhushan, Methods and compositions for analyzing nucleic acids.
    상세보기
  22. Lasken, Roger S.; Egholm, Michael; Alsmadi, Osama A., Nucleic acid amplification.
    상세보기
  23. Metzger, Steven W.; Hance, Kenneth Robert, Rapid cell purification systems.
    상세보기
  24. Metzger, Steven W.; Hance, Kenneth Robert, Rapid cell purification systems.
    상세보기
  25. Metzger, Steven W.; Hance, Kenneth Robert, Rapid cell purification systems.
    상세보기
  26. Shamsheyeva, Alena; Howson, David C.; Metzger, Steven W., Rapid determination of microbial growth and antimicrobial susceptibility.
    상세보기
  27. Metzger, Steven W.; Howson, David C.; Goldberg, David A.; Buttry, Daniel A., Rapid microbial detection and antimicrobial susceptibility testing.
    상세보기
  28. Korfhage, Christian; Peist, Ralf; Löffert, Dirk, Reverse transcription and amplification of RNA with simultaneous degradation of DNA.
    상세보기
  29. Nanibhushan Dattagupta ; C. Nagaraja Sridhar ; Whei-Kuo Wu, Sample processing to release nucleic acids for direct detection.
    상세보기
  30. Goldberg, David A.; Howson, David C.; Metzger, Steven W.; Buttry, Daniel A.; Saavedra, Steven Scott, Sensitive and rapid determination of antimicrobial susceptibility.
    상세보기
  31. Goldberg, David A.; Howson, David C.; Metzger, Steven W.; Buttry, Daniel A.; Saavedra, Steven Scott, Sensitive and rapid determination of antimicrobial susceptibility.
    상세보기
  32. Abarzúa, Patricio; Smelkova, Natalia; Sparkowski, Jason, Universal reagents for rolling circle amplification and methods of use.
    상세보기

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