초록 ▼

Analytical test kits and assay procedure employs test cards or strips of an inert plastic carrier having a flat, non-absorbent surface, containing thereon spots of insolubilized reagent for the determination of an analyte, such as an antigen or an antibody, in a liquid medium such as serum or other

Analytical test kits and assay procedure employs test cards or strips of an inert plastic carrier having a flat, non-absorbent surface, containing thereon spots of insolubilized reagent for the determination of an analyte, such as an antigen or an antibody, in a liquid medium such as serum or other body fluids in a solid phase enzyme immunoassay of the sandwich type. Different binding partners can be placed as separate spots on the same card for the assay of a group of related or unrelated analytes in the same test sample. An insoluble colored product on a spot, qualitatively and quantitatively, is indicative of a positive result.

대표청구항 ▼

[ We claim:] [1.]1. A test kit for detecting the presence in a sample of at least one component to be detected selected from the group consisting of antigens, antibodies and hastens by a sandwich enzyme immunoassay comprising in one or more containers:a. an analytical device consisting essentially o

[ We claim:] [1.]1. A test kit for detecting the presence in a sample of at least one component to be detected selected from the group consisting of antigens, antibodies and hastens by a sandwich enzyme immunoassay comprising in one or more containers:a. an analytical device consisting essentially of an inert, opaque plastic carrier having a flat, hydrophobic surface that is non-absorbent to said antigens, antibodies, hastens and to enzymes, having a multiplicity of visually locatable discrete locations and, deposited directly on said surface within at least one of said discrete locations, a spot of an insolubilized specific first binding partner of the at least one component to be detected;b. a working reagent conjugate of predetermined concentration of at least about 15 micrograms per ml in liquid form comprising an enzyme coupled to a specific second binding partner of the at least one component to be detected; andc. an enzyme substrate in liquid form comprising a material capable of reacting with said enzyme at room temperature to form a colored precipitate detectable by the naked eye against said opaque carrier.