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[미국특허] Methods and compositions for polypeptide engineering 원문보기

IPC분류정보
국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판)
  • C12Q-001/68
  • C12P-019/34
  • C12N-001/20
  • C12N-005/00
  • C07H-021/02
출원번호 US-0339913 (1999-06-24)
발명자 / 주소
  • Patten Phillip A.
  • Stemmer Willem P.C.
출원인 / 주소
  • Maxygen, Inc.
대리인 / 주소
    Kruse
인용정보 피인용 횟수 : 78  인용 특허 : 53

초록

Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed.

대표청구항

[ What is claimed is:] [1.]1. A method for selecting or screening an evolved DNA substrate molecule encoding an interferon alpha, comprising:(a) providing a library of mutant alpha interferon genes, the library comprising an expression vector wherein the alpha interferon genes are expressed under th

이 특허에 인용된 특허 (53) 인용/피인용 타임라인 분석

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  3. Waldmann Herman (University of Cambridge ; Department of Pathology ; Immunology Div. ; Tennis Court Road Cambridge ; CB2 1QP GB3) Walsh Louise (The University of Cambridge ; Department of Pathology ;, CDR grafted humanised chimeric T-cell antibodies.
  4. Goodman Myron F. (4719 Alminar Ave. LaCanada CA 91011) Reha-Krantz Linda J. (10044 87th Avenue Edmonton ; Alberta CAX T6E 2N9 ), Chain-terminating nucleotides for DNA sequencing methods.
  5. Weiner David ; Williams William ; Levy David N., Chimeric envelope proteins for viral targeting.
  6. Tom Henry K. (La Honda CA) Rowley Gerald L. (Cupertino CA), Concentrating zone method in heterogeneous immunoassays.
  7. Stemmer Willem P. C. ; Crameri Andreas, DNA mutagenesis by random fragmentation and reassembly.
  8. Sninsky John J. (El Sobrante CA) Kwok Shirley Y. (San Ramon CA) Mack David H. (Berkeley CA) Ehrlich Henry A. (Oakland CA) Mullis Kary B. (LaJolla CA), Detection of viruses by amplification and hybridization.
  9. Stemmer Willem P. C. ; Lipshutz Robert J., End-complementary polymerase reaction.
  10. Stemmer Willem Peter Christiaan ; Lipshutz Robert J., End-complementary polymerase reaction.
  11. Stemmer Willem P. C. (Carlsbad CA), Enzymatic inverse polymerase chain reaction library mutagenesis.
  12. Ullman Edwin F. (Atherton CA) Schwarzberg Moshe (Palo Alto CA), Fluorescence quenching with immunological pairs in immunoassays.
  13. Kronick Melvyn N. (Palo Alto CA) Little William A. (Palo Alto CA), Fluorescent immunoassay employing total reflection for activation.
  14. Gray Gregory L. (South San Francisco CA), Hybrid prokaryotic polypeptides produced by in vivo homologous recombination.
  15. Maggio Edward T. (Redwood City CA), Kit for carrying out chemically induced fluorescence immunoassay.
  16. Litman David J. (Palo Alto CA) Harel Zvi (Stanford CA) Ullman Edwin F. (Atherton CA), Macromolecular environment control in specific receptor assays.
  17. Stemmer Willem P. C., Method for directing evolution of a virus.
  18. Ho Steffan N. (Rochester MN) Horton Robert M. (Rochester MN), Method for gene splicing by overlap extension using the polymerase chain reaction.
  19. Grandi Guido (Segrate ITX) De Ferra Francesca (Lodi ITX) Galli Giuliano (Monterotondo ITX) Cosmina Paola (Milan ITX), Method for genetically manipulating peptide synthetases.
  20. Goeddel David V. (Hillsborough CA) Rice Glenn C. (Palo Alto CA) Leung David W. H. (Foster City CA), Method for making variant secreted proteins with altered properties.
  21. Dubensky ; Jr. Thomas W. ; Polo John M. ; Chang Steven M.W. ; Jolly Douglas J., Method for stimulating an immune response utilizing recombinant alphavirus particles.
  22. Short Jay M., Method of DNA reassembly by interrupting synthesis.
  23. Kauffman Stuart Alan ; Ballivet Marc,CHX, Method of identifying a stochastically-generated peptide, polypeptide, or protein having ligand binding property and co.
  24. Minshull Jeremy ; Stemmer Willem P. C., Methods and compositions for cellular and metabolic engineering.
  25. Stemmer Willem P. C., Methods for generating polynucleotides having desired characteristics by iterative selection and recombination.
  26. Stemmer Willem P. C. ; Crameri Andreas, Methods for generating polynucleotides having desired characteristics by iterative selection and recombination.
  27. Stemmer Willem P. C. (Los Gatos CA), Methods for in vitro recombination.
  28. Stemmer Willem P. C., Methods of shuffling polynucleotides.
  29. Prasher Douglas (Falmouth MA), Modified apoaequorin having increased bioluminescent activity.
  30. Dahlberg James E. (Madison WI) Lyamichev Victor I. (Madison WI) Brow Mary Ann D. (Madison WI), Nucleic acid encoding synthesis-deficient thermostable DNA polymerase.
  31. Wu Kun C. (Omaha NE), PCR-based strategy of constructing chimeric DNA molecules.
  32. Sorge Joseph A. (Rancho Santa Fe CA) Mullinax Rebecca L. (San Diego CA), Polymerase compositions and uses thereof.
  33. Raikhel Natasha V. (Okemos MI), Polypeptides enabling sorting of proteins to vacuoles in plants.
  34. Crowe James Scott,GB2 ; Lewis Alan Peter,GB2, Preparation of chimaeric antibodies using the recombinant PCR strategy.
  35. Desrosiers Ronald C., Primate lentivirus antigenic compositions.
  36. Mullis Kary B. (Kensington CA), Process for amplifying nucleic acid sequences.
  37. Mullis Kary B. (La Jolla CA) Erlich Henry A. (Oakland CA) Arnheim Norman (Woodland Hills CA) Horn Glenn T. (Emeryville CA) Saiki Randall K. (Richmond CA) Scharf Stephen J. (Berkeley CA), Process for amplifying, detecting, and/or cloning nucleic acid sequences.
  38. Mullis Kary B. (La Jolla CA) Erlich Henry A. (Oakland CA) Gelfand David H. (Oakland CA) Horn Glenn (Emeryville CA) Saiki Randall K. (Richmond CA), Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme.
  39. Honjo Tasuku (Kanyu-chi ; Oiwake-cho ; Kita-Shirakawa Sakyo-ku ; Kyoto ; Kyoto JPX) Tashiro Kei (Kyoto JPX) Tada Hideaki (Mishima JPX), Process for constructing cDNA library, and novel polypeptide and DNA coding for the same.
  40. Kauffman Stuart Alan ; Ballivet Marc,CHX, Process for obtaining DNA, RNA, peptides, polypeptides, or protein, by recombinant DNA technique.
  41. Kauffman Stuart Alan ; Ballivet Marc,CHX, Process for obtaining DNA, RNA, peptides, polypeptides, or proteins, by recombinant DNA technique.
  42. Kauffman Stuart Alan ; Ballivet Marc,CHX, Process for the production of expression vectors comprising at least one stochastic sequence of polynucleotides.
  43. Kauffman Stuart Alan ; Ballivet Marc,CHX, Process for the production of stochastically-generated peptides,polypeptides or proteins having a predetermined propert.
  44. Kauffman Stuart Alan ; Ballivet Marc,CHX, Process for the production of stochastically-generated transcription or translation products.
  45. Hoogenboom Hendricus R. J. M. (Cambridge GBX) Baier Michael (Frankfurt DEX) Jespers Laurent S. A. T. (Tervuren BEX) Winter Gregory P. (Cambridge GBX), Production of chimeric antibodies - a combinatorial approach.
  46. Short Jay M., Production of enzymes having desired activities by mutagenesis.
  47. Short Jay M., Protein activity screening of clones having DNA from uncultivated microorganisms.
  48. Mullis Kary B. (La Jolla CA), Rapid purification of DNA.
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  50. Arnold Frances H. (Pasadena CA) Chen Keqin (Pasadena CA), Subtilisin variants suitable for hydrolysis and synthesis in organic media.
  51. Varadaraj Ramesh (Flemington NJ) Brons Cornelius H. (Washington NJ) Bock Jan (Warren NJ) Brois Stanley J. (Westfield NJ), Surfactant-nutrients for bioremediation of hydrocarbon contaminated soils and water.
  52. Tabor Stanley (Cambridge MA) Richardson Charles C. (Chestnut Hill MA), T7 DNA polymerase.
  53. Davis Maria ; Moffett R. Bruce ; Fuller Carl W. ; Cunniff John J., Thermostable DNA polymerases.

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