초록 ▼

An efficient method for the microfabrication of electronic devices which have been adapted for the analyses of biologically significant analyte species is described. The techniques of the present invention allow for close control over the dimensional features of the various components and layers est

An efficient method for the microfabrication of electronic devices which have been adapted for the analyses of biologically significant analyte species is described. The techniques of the present invention allow for close control over the dimensional features of the various components and layers established on a suitable substrate. Such control extends to those parts of the devices which incorporate the biological components which enable these devices to function as biological sensors. The materials and methods disclosed herein thus provide an effective means for the mass production of uniform wholly microfabricated biosensors. Various embodiments of the devices themselves are described herein which are especially suited for real time analyses of biological samples in a clinical setting. In particular, the present invention describes assays which can be performed using certain ligand/ligand receptor-based biosensor embodiments. The present invention also discloses a novel method for the electrochemical detection of particular analyte species of biological and physiological significance using an substrate/label signal generating pair which produces a change in the concentration of electroactive species selected from the group consisting of dioxygen and hydrogen peroxide.

대표청구항 ▼

[ What is claimed is:] [1.]1. A method of forming a patterned layer on a substantially planar surface, comprising(a) microdispensing, at a predetermined location, an amount of a photoformable matrix onto said planar surface sufficient to cover a predetermined area having a defined perimeter and to e

[ What is claimed is:] [1.]1. A method of forming a patterned layer on a substantially planar surface, comprising(a) microdispensing, at a predetermined location, an amount of a photoformable matrix onto said planar surface sufficient to cover a predetermined area having a defined perimeter and to extend beyond said area and perimeter, forming a microdispensed matrix, wherein the area covered by the microdispensed matrix is less than the entire area of the planar surface;(b) drying said microdispensed matrix;(c) aligning said microdispensed matrix with a mask of the predetermined area and defined perimeter at the predetermined location to form an aligned matrix;(d) exposing said aligned matrix to radiation to form an exposed matrix; and(e) developing said exposed matrix to remove excess microdispensed photoformable matrix, such that a patterned layer is formed at the predetermined location, said patterned layer having the predetermined area and defined perimeter.