[특허]Multi-well microfiltration apparatus

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	B01D-061/00 B01D-063/08
출원번호	US-0565566 (2000-05-04)
발명자 / 주소	Kevin S. Bodner Alfred P. Madden Joseph Jackson Jason H. Halsey Mark T. Reed Ward Frye Mark F. Oldham Stephen E. Moring Jon Hoshizaki
출원인 / 주소	PE Corporation (NY)
대리인 / 주소	Kilyk & Bowersox, P.L.L.C.
인용정보	피인용 횟수 : 41 인용 특허 : 45

초록 ▼

The present invention provides multi-well plates and column arrays in which samples (e.g., cell lysates containing nucleic acids of interest, such as RNA) can be analyzed and/or processed. In one embodiment, the microfiltration arrangement is a multilayer structure, including (i) a column plate havi

The present invention provides multi-well plates and column arrays in which samples (e.g., cell lysates containing nucleic acids of interest, such as RNA) can be analyzed and/or processed. In one embodiment, the microfiltration arrangement is a multilayer structure, including (i) a column plate having an array of minicolumns into which samples can be placed, (ii) a discrete filter element disposed in each minicolumn, (iii) a drip-director plate having a corresponding array of drip directors through which filtrate may egress, and (iv) a receiving-well plate having a corresponding array of receiving wells into which filtrate can flow. The invention provides multi-well microfiltration arrangements that are relatively simple to manufacture and that overcome many of the problems associated with the prior arrangements relating to (i) cross-contamination due to wicking across a common filter sheet or (ii) individual filter elements entrapping sample constituents within substantial dead volumes. Further, the invention provides multi-well microfiltration arrangements that adequately support discrete filter elements disposed in the wells without creating substantial preferential flow. Additionally, the invention provides multi-well microfiltration arrangements that avoid cross-contamination due to aerosol formation, pendent drops and/or splattering. Other disclosed features of the invention provide for the automated covering or heat-sealing of filtrate samples separately collected in an array of wells.

대표청구항 ▼

1. A microfiltration apparatus for processing a plurality of fluid samples, comprising:a first plate having a plurality of columns, each column containing at one end thereof a filter element and a fluid discharge conduit beneath the filter element; a second plate spaced apart from said first plate b

1. A microfiltration apparatus for processing a plurality of fluid samples, comprising:a first plate having a plurality of columns, each column containing at one end thereof a filter element and a fluid discharge conduit beneath the filter element; a second plate spaced apart from said first plate by a cavity, said second plate having (i) a plurality of collection wells aligned with said columns for receiving sample fluid from said discharge conduits, and (ii) a plurality of vents extending through the second plate adjacent said collection wells; a gas-permeable material positioned in said cavity between the first plate and the second plate, said material laterally surrounding the region between at least one discharge conduit and an aligned collection well; wherein said gas-permeable material is effective (i) to permit a vacuum drawn from beneath said second plate to extend, via said vents, to a region above said second plate and to said plurality of columns, thereby drawing fluid from said columns into said collection wells and (ii) to obstruct movement of aerosols across the top of said second plate, thereby discouraging cross-contamination between wells.

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이 특허에 인용된 특허 (45) 인용/피인용 타임라인 분석

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Clark Phillip (Malden MA) Lee Carolyn (Sudbury MA) Rising Donald B. (Stow MA) Zermani Thomas (Concord MA), Multi-well filtration test apparatus.
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Lyman George (Cape Porpoise ME) Labriola Anthony (Woburn MA), Multi-well test plate.
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Valus Ronald J. (Valley View OH) Lipsky Jonathan N. (Hanover MA), Process for sealing microplates utilizing a thin polymeric film.
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