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A recombinant baculovirus, which produces a recombinant polyhedra made up of a baculovirus polyhedrin (PH), a Bacillus thuringiensis crystal protein (CP) and jellyfish Aequorea victoria green fluorescent protein (GFP), is constructed by introducing a transfer vector carrying a fusion gene encoding a

A recombinant baculovirus, which produces a recombinant polyhedra made up of a baculovirus polyhedrin (PH), a Bacillus thuringiensis crystal protein (CP) and jellyfish Aequorea victoria green fluorescent protein (GFP), is constructed by introducing a transfer vector carrying a fusion gene encoding a fusion protein in which the PH, the CP and the GFP are directly linked from N-terminal to C-terminal, in sequence, and a wild-type baculovirus into an insect cell, simultaneously, and culturing the cell. This baculovirus transfer vector pColorBtrus is constructed by synthesizing the GFP-coding DNA fragment from plasmid pGFP, the PH gene from wild-type Autographa californica Nucleopolyhedrovirus, and a Cry1Ac gene from plasmid pPN6.6 carrying a Bacillus thuringiensis Cry1Ac gene by polymerase chain reactions, and inserting first the GFP-coding DNA fragment and the PH gene in baculovirus expression vector pAcUW31 in such a way that the 5'-end of the GFP-coding DNA fragment is linked to the 3'-end of the PH gene and then, the Cry1Ac gene between the PH gene and the GFP-coding DNA fragment. The recombinant virus can kill insect pests with high pathogenicity within a short time, and is equipped with a monitoring device for infected insects, so that it can be prevented from being overused.

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1. A recombinant baculovirus comprising a genome wherein the genome comprises two independent transcription units:wherein the first transcription unit comprises a nucleic acid sequence encoding a functional polyhedrin protein capable of forming a paracrystalline lattice around the viral particle ope

1. A recombinant baculovirus comprising a genome wherein the genome comprises two independent transcription units:wherein the first transcription unit comprises a nucleic acid sequence encoding a functional polyhedrin protein capable of forming a paracrystalline lattice around the viral particle operably linked to promoter; and, wherein the second transcription unit transcribes a fusion gene wherein the fusion gene comprises a nucleic acid sequence encoding a polyhedrin, a gfp nucleic acid sequence encoding a green fluorescent protein of a jellyfish Aequorea victoria and nucleic acid sequence encoding a Bacillus thuringiensis crystal protein, or fragment thereof, having insecticidal activity and in which the nucleic acid sequences are sequentially linked and are operably linked to a polyhedrin promoter so that infectious occlusion bodies with the viral particle produced from said baculovirus contains a fusion gene product, wherein the fusion gene product encodes a fusion protein wherein the baculovirus polyhedron protein, Bacillus thuringiensis crystal protein and jellyfish Aequorea victoria green fluorescent protein are sequentially linked from N-terminal to C-terminal.