[특허]Methods for gene expression monitoring on electronic microarrays

[미국특허] Methods for gene expression monitoring on electronic microarrays 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	C12Q-001/68 C12P-019/34
출원번호	US-0710200 (2000-11-09)
발명자 / 주소	Elaine M. Weidenhammer Ling Wang Xiao Xu Michael J. Heller Brenda F. Kahl
출원인 / 주소	Nanogen, Inc.
대리인 / 주소	Lyon & Lyon LLP
인용정보	피인용 횟수 : 42 인용 특허 : 2

초록 ▼

The present invention presents methods for gene expression monitoring that utilize microelectronic arrays to drive the transport and hybridization of nucleic acids. Procedures are described for generating mRNA expression samples for use in these methods from populations of cells, tissues, or other biological source materials, that may differ in their physiological and/or pathological state. Provided in the invention are methods for generating a reusable nucleic acid transcript library from mRNA in a sample of biological material. In order to improve gene expression monitoring on the microelectronic arrays, the transcripts are amplified to produce sample nucleic acid amplicons of a defined length. Because multiple sample amplicons may be selectively hybridized to controlled sites in the electronic array, the gene expression profiles of the polynucleotide populations from different sources can be directly compared in an array format using electronic hybridization methodologies. Also provided in the invention are methods for detecting the level of sample amplicons using electronically assisted primer extension detection, and utilizing individual test site hybridization controls. The hybridization data collected utilizing the improved methods of the present invention will allow the correlation of changes in mRNA level with the corresponding expression of the encoded protein in the biological source material, and thus aid in studying the role of gene expression in disease.

대표청구항 ▼

1. A method for detecting the relative amounts of at least two different mRNA sequences in at least one biological sample, the method comprising:(a) isolating mRNA from at least one biological sample; (b) amplifying at least two mRNA transcripts from each biological sample to produce amplicons, wherein the amplicons are less than about 300 bases in length, and wherein the amplification comprises a linear amplification step; (c) electronically hybridizing the amplicons produced in step (b) to at least two probes bound to a support at predetermined locations; and (d) detecting the amounts of each amplicon hybridized to the bound probes at the predetermined locations.

이 특허에 인용된 특허 (2) 인용/피인용 타임라인 분석

Heller Michael J. (Encinitas CA) Tu Eugene (San Diego CA), Active programmable electronic devices for molecular biological analysis and diagnostics.
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Hu Qianjin ; Peng Allan, Proportional amplification of mRNA from a linear template in vitro.
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이 특허를 인용한 특허 (42) 인용/피인용 타임라인 분석

El Gamal, Abbas; Eltoukhy, Helmy; Salama, Khaled, Biological analysis arrangement and approach therefor.
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El Gamal, Abbas; Eltoukhy, Helmy; Salama, Khaled, Biological analysis arrangement and approach therefor.
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Pisharody, Sobha M.; Kunwar, Sandeep; Mathai, George T., Electronic detection of biological molecules using thin layers.
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Richards, William L.; Ashby, Austin; Ketterer, Matthew; Marshall, Kevin; Harrison, Josh; Mette, Matthew; Richards, Paul; Showalter, Wayne; Cote, Jasmin; Halbert, Phillip C.; Bourgeois, Solene; Metzger, Steven W.; Hance, Ken; Mensack, Meghan; Michel, Carlos; Allers, Elke; Gamage, Dulini; Prisbrey, Landon; Gusyatin, Oleg; Shamsheyeva, Alena; Turng, Ben; Ghusson, Andrew; Reinhardt, Kurt, Instrument and system for rapid microorganism identification and antimicrobial agent susceptibility testing.
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Mohapatra, Shyam S.; Behera, Aruna K., Materials and methods for prevention and treatment of RNA viral diseases.
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Mohapatra, Shyam S.; Behera, Aruna K., Materials and methods for prevention and treatment of RNA viral diseases.
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Prisbrey, Landon; Blackmore, Elise; Hance, Kenneth R.; Metzger, Steven W.; Marshall, Kevin, Membrane-assisted purification.
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McGarry, Mark W.; Kahn, Peter; Tuggle, Todd; Hawkins, George, Method and apparatus for performing biological reactions on a substrate surface.
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Berrada, Abdelkrim; Liang, MingHwa Benjamin; Jung, Lawrence, Method and device for marking articles.
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Choong, Vi-En; Li, Changming; Shi, Song; Maracas, George, Method for enhanced bio-conjugation events.
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Liu, Xuanbin; Pan, Tao; Sun, Zhenhong, Microarray reader based on evanescent wave detection.
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Zheng, Yuan; Xu, Leon; Sun, Zhenhong, Microarray reader based on evanescent wave detection and method of reading a microarray.
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Murrah, Judith; Tran, Phidung H.; Szczepanik, Maciej B.; Liang, MingHwa Benjamin; Hayward, James A., Multimode image and spectral reader.
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Li, Changming; Sawyer, Jaymie Robin; Choong, Vi-En; Maracas, George; Zhang, Peiming, Protein and peptide sensors using electrical detection methods.
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Allen, Nadia; Qiu, Peter, Quantitative helicase assay.
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Metzger, Steven W.; Hance, Kenneth Robert, Rapid cell purification systems.
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Shamsheyeva, Alena; Howson, David C.; Metzger, Steven W., Rapid determination of microbial growth and antimicrobial susceptibility.
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Metzger, Steven W.; Howson, David C.; Goldberg, David A.; Buttry, Daniel A., Rapid microbial detection and antimicrobial susceptibility testing.
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Metzger,Steven W.; Howson,David C.; Goldberg,David A.; Buttry,Daniel A., Rapid microbial detection and antimicrobial susceptibility testing.
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Gong,Haiqing; Yap,Eric Peng Huat; Chen,Longqing, Sample preparation integrated chip.
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Goldberg, David A.; Howson, David C.; Metzger, Steven W.; Buttry, Daniel A.; Saavedra, Steven Scott, Sensitive and rapid determination of antimicrobial susceptibility.
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Goldberg, David A.; Howson, David C.; Metzger, Steven W.; Buttry, Daniel A.; Saavedra, Steven Scott, Sensitive and rapid determination of antimicrobial susceptibility.
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Goldberg, David A.; Howson, David C.; Metzger, Steven W.; Buttry, Daniel A.; Saavedra, Steven Scott, Sensitive and rapid determination of antimicrobial susceptibility.
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Goldberg, David A.; Howson, David C.; Metzger, Steven W.; Buttry, Daniel A.; Saavedra, Steven Scott, Sensitive and rapid determination of antimicrobial susceptibility.
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Hellyer, Tobin; Fort, Thomas; McMillian, Ray A., Use of an extraction control in a method of extracting nucleic acids.
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Hellyer,Tobin; Fort,Thomas; McMillian,Ray A., Use of an extraction control in a method of extracting nucleic acids.
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[미국특허] Methods for gene expression monitoring on electronic microarrays 원문보기