초록 ▼

A micro-machined nuclear transfer array (NTA) provides high-throughput transfer of nuclei between two cells. Donor cells containing the nuclei to be transferred are placed in microwells positioned adjacent to microwells containing the recipient biological cell. The microwells are contained within an

A micro-machined nuclear transfer array (NTA) provides high-throughput transfer of nuclei between two cells. Donor cells containing the nuclei to be transferred are placed in microwells positioned adjacent to microwells containing the recipient biological cell. The microwells are contained within an upper chamber patterned with parallel rows of microwells of the diameter of the cell of interest. An injection port is formed in the bottom of the microwells through which nuclei may pass during transfer. The NTA also contains a lower chamber having a second array of capture wells of similar dimension and in register with the upper chamber for receiving the nuclei removed from the cell in the upper chamber. Nuclei are removed from the donor cells in the upper chamber, transferred to capture wells in the lower chamber, and inserted into the recipient biological cells of the upper chamber. The upper tray moves relative to the lower chamber so that the nuclei in the capture well are transferred to the recipient biological cell to complete the nuclear transfer process.

대표청구항 ▼

1. A nuclear transfer array for simultaneously transferring a plurality of nuclei from a plurality of donor biological cells to a plurality of recipient biological cells comprising:a) an upper chamber containing a plurality of rows of microwells for receiving a plurality of donor biological cells an

1. A nuclear transfer array for simultaneously transferring a plurality of nuclei from a plurality of donor biological cells to a plurality of recipient biological cells comprising:a) an upper chamber containing a plurality of rows of microwells for receiving a plurality of donor biological cells and a plurality of recipient biological cells in alternating ones of said rows, each of said microwells having a port formed in a bottom thereof; b) a lower chamber selectively positioned beneath said upper chamber, said lower chamber containing a plurality of capture wells each having a bottom wall, wherein said capture wells are selectively positionable beneath said ports in said microwells of said upper chamber and movement of said nuclei between said upper chamber and said lower chamber.