초록 ▼

A cartridge (101) for separating a desired analyte from a fluid sample includes a sample port (103) and a sample flow path extending from the port through the body of the cartridge. The sample flow path includes at least one flow-through component (122), e.g., filter paper or a microfabricated chip,

A cartridge (101) for separating a desired analyte from a fluid sample includes a sample port (103) and a sample flow path extending from the port through the body of the cartridge. The sample flow path includes at least one flow-through component (122), e.g., filter paper or a microfabricated chip, for capturing the desired analyte from the sample as the sample flows through the cartridge. The cartridge also includes an elution flow path for carrying elution fluid through the component (122) to release captured analyte from the component into the elution fluid. The elution flow path diverges from the sample flow path after passing through the component (122). Flow controllers (41A) and (41B) direct the remaining fluid sample into a waste chamber (139) after the sample flows through the component (122) and direct the elution fluid and eluted analyte into a reagent chamber (141) and reaction chamber (143). The continuous-flow design of the cartridge permits the rapid processing of a fluid sample that is larger in volume than any interactive region within the cartridge, allowing increased sensitivity in the detection of low copy concentrations of analytes, such as nucleic acid.

대표청구항 ▼

1. A device for use with an ultrasonic transducer to separate an analyte from a fluid sample, the device comprising a cartridge having:a) a first flow path including: i) a lysing chamber for lysing sample components to release the analyte therefrom, wherein the lysing chamber contains a membrane or

1. A device for use with an ultrasonic transducer to separate an analyte from a fluid sample, the device comprising a cartridge having:a) a first flow path including: i) a lysing chamber for lysing sample components to release the analyte therefrom, wherein the lysing chamber contains a membrane or filter for capturing the sample components as the sample flows through the lysing chamber, and wherein the lysing chamber is defined by at least one wall for contacting the transducer to effect the transfer of ultrasonic energy to the lysing chamber; ii) an extraction chamber, downstream of the lysing chamber, the extraction chamber containing at least one solid support for capturing the released analyte; and iii) at least a first waste chamber, downstream of the extraction chamber, for receiving waste fluid; b) a second flow path for eluting the captured analyte from the extraction chamber, wherein the second flow path passes through the extraction chamber and diverges from the first flow path after passing through the extraction chamber; and c) at least one flow controller for directing the waste fluid into the waste chamber and for directing the eluted analyte into the second flow path.