초록 ▼

A process of producing somatic seedlings from a somatic embryo. The process comprising pre-germinating somatic embryos, placing the pre-germinated somatic embryos into a state of physiological dormancy, sowing the pre-germinated physiologically dormant somatic embryos onto or into germination media,

A process of producing somatic seedlings from a somatic embryo. The process comprising pre-germinating somatic embryos, placing the pre-germinated somatic embryos into a state of physiological dormancy, sowing the pre-germinated physiologically dormant somatic embryos onto or into germination media, and propagating the sown pre-germinated somatic embryos in environmental conditions manipulated to facilitate imbibition, germination, and development into complete seedlings possessing shoots and roots. Advantageously, the process may be carried out with "naked" embryos (i.e., non-encapsulated or otherwise coated embryos) using conventional seed handling equipment, growing mixes, and plant propagation environments. The process does not require the use of aseptic techniques or sterilized media or equipment. The invention also relates to pre-germinated embryos and seedlings produced by the process.

대표청구항 ▼

1. A process of producing a somatic seedling from a somatic plant embryo, said process comprising the steps of:(a) pre-germinating a somatic embryo by placing the somatic embryo in contact with a liquid medium used for germinating somatic embryos to produce a pre-germinated somatic embryo, (b) optio

1. A process of producing a somatic seedling from a somatic plant embryo, said process comprising the steps of:(a) pre-germinating a somatic embryo by placing the somatic embryo in contact with a liquid medium used for germinating somatic embryos to produce a pre-germinated somatic embryo, (b) optionally contacting the partially germinated somatic embryo with a solution of abscisic acid (ABA), (c) drying the pre-germinated somatic embryo, (d) placing the dried pre-germinated somatic embryo on or within the surface of a non-sterile three-phase substrate, said phases comprising solid, liquid and gas phases, (e) placing said substrate containing said dried pre-germinated somatic embryo into an environmentally-controlled plant-growing environment, (f) controlling at least one environmental factor in said environment during germination of the dried pre-germinated somatic embryo to facilitate ex vitro re-germination, growth and development of the dried pre-germinated somatic embryo into a normal somatic seedling possessing shoot and root, and (g) applying water and/or nutrient solutions at regular intervals during said period of somatic embryo re-germination to the surface of the substrate in the form of microdroplets or by irrigating or drenching the three-phase substrate such that said ex vitro re-germination, growth and development of said dried pre-germinated somatic embryo occur to produce said normal somatic seedling possessing shoot and root.