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Kafe 바로가기국가/구분 | United States(US) Patent 등록 |
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국제특허분류(IPC7판) |
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출원번호 | US-0427808 (1999-10-27) |
발명자 / 주소 |
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출원인 / 주소 |
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대리인 / 주소 |
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인용정보 | 피인용 횟수 : 875 인용 특허 : 257 |
A sensor utilizing a non-leachable or diffusible redox mediator is described. The sensor includes a sample chamber to hold a sample in electrolytic contact with a working electrode, and in at least some instances, the sensor also contains a non-leachable or a diffusible second electron transfer agen
A sensor utilizing a non-leachable or diffusible redox mediator is described. The sensor includes a sample chamber to hold a sample in electrolytic contact with a working electrode, and in at least some instances, the sensor also contains a non-leachable or a diffusible second electron transfer agent. The sensor and/or the methods used produce a sensor signal in response to the analyte that can be distinguished from a background signal caused by the mediator. The invention can be used to determine the concentration of a biomolecule, such as glucose or lactate, in a biological fluid, such as blood or serum, using techniques such as coulometry, amperometry, and potentiometry. An enzyme capable of catalyzing the electrooxidation or electroreduction of the biomolecule is typically provided as a second electron transfer agent.
1. An electrochemical sensor strip for determining the concentration of glucose in blood or interstitial fluid, the sensor strip comprising:(a) a first inert, non-conducting polymeric base layer and a second inert, non-conducting polymeric base layer, each layer having a side length and a shorter en
1. An electrochemical sensor strip for determining the concentration of glucose in blood or interstitial fluid, the sensor strip comprising:(a) a first inert, non-conducting polymeric base layer and a second inert, non-conducting polymeric base layer, each layer having a side length and a shorter end length; (b) a spacer material positioned between and connecting the first and second base layers, the spacer material shaped to define a channel between the layers, the spacer defining: (i) a first opening at a first end of the sensor strip; (ii) a second opening at a first side edge of the sensor strip; and (iii) a sample chamber adjacent the first opening; (c) an electrode pair comprising a first working electrode and a counter electrode, each formed on one of the base layers and exposed to the sample chamber, at least a portion of the first working electrode being within 200 .mu.m of a portion of the counter electrode; (d) a measurement zone adjacent the first working electrode and counter electrode within the sample chamber, the measurement zone sized to contain a volume of no more than 1 .mu.L of sample; (e) redox mediator disposed in the sample chamber; (f) an indicator electrode that is a second working electrode formed on the same base layer as the first working electrode and the counter electrode, the indicator electrode: (i) exposed to the sample chamber; and (ii) positioned farther along the channel from the first opening than the first working electrode; and (g) a conductive trace extending from each of the electrodes along a length of the base layer and extending to a position adjacent a second end length of the sensor strip.
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