초록 ▼

A biosensor (10) has a hydrogel (30) in a rigid and referably biocompatible enclosure (20). The hydrogel (30) includes an immobilized glucose-binding molecule such as concanavalin A (Con A) and an immobilized hexose saccharide such as a-D-mannopyranoside. The immobilized hexose saccharide competitiv

A biosensor (10) has a hydrogel (30) in a rigid and referably biocompatible enclosure (20). The hydrogel (30) includes an immobilized glucose-binding molecule such as concanavalin A (Con A) and an immobilized hexose saccharide such as a-D-mannopyranoside. The immobilized hexose saccharide competitively binds with free glucose to the glucose-binding molecules, thus changing the number of crosslinks in the hydrogel (30), which changes hydrogel swelling tendency and the pressure of the hydrogel in its confined space in proportion to the concentration of free glucose. By measuring the change in hydrogel pressure with a pressure transducer (40), the biosensor (10) is able to accurately measure the concentration of the tree glucose molecule without the problem of oxygen limitations and interference encountered by prior art biosensors. A battery (64) powered telemeter (60) operably engaged to the pressure transducer (40) sends a radio data signal to a receiver (66) containing an alarm system operably attached to a computer (62). Furthermore, an alarm system utilizes such a sensor to automatically notify a person that the blood glucose level is outside a predetermined parameter, and/or to automatically inject an agent such as glucose or glycogen which will raise blood glucose levels.

대표청구항 ▼

1. A biosensor for measuring the concentration of glucose, the biosensor comprising:a polymeric hydrogel that changes its crosslinking density and swelling tendency in proportion to the concentration of free glucose; glucose binding molecules chemically or physically immobilized in the hydrogel; a h

1. A biosensor for measuring the concentration of glucose, the biosensor comprising:a polymeric hydrogel that changes its crosslinking density and swelling tendency in proportion to the concentration of free glucose; glucose binding molecules chemically or physically immobilized in the hydrogel; a hexose saccharide or a polysaccharide chemically or physically immobilized in the hydrogel, the free glucose competitively binds with the immobilized hexose saccharide or polysaccharide to the glucose binding molecules, which reduces the number of hydrogel crosslinks, thereby causing the hydrogel to swell and increasing the pressure exerted by the hydrogel in its enclosure; a means for measuring the pressure or amount of swelling of the hydrogel; and a means for reporting the concentration of the glucose molecule based on the measured pressure of the hydrogel.