초록 ▼

Libraries of unimolecular, double-stranded oligonucleotides on a solid support. These libraries are useful in pharmaceutical discovery for the screening of numerous biological samples for specific interactions between the double-stranded oligonucleotides, and peptides, proteins, drugs and RNA. In a

Libraries of unimolecular, double-stranded oligonucleotides on a solid support. These libraries are useful in pharmaceutical discovery for the screening of numerous biological samples for specific interactions between the double-stranded oligonucleotides, and peptides, proteins, drugs and RNA. In a related aspect, the present invention provides libraries of conformationally restricted probes on a solid support. The probes are restricted in their movement and flexibility using double-stranded oligonucleotides as scaffolding. The probes are also useful in various screening procedures associated with drug discovery and diagnosis. The present invention further provides methods for the preparation and screening of the above libraries.

대표청구항 ▼

1. A synthetic conformationally-restricted probe library comprising a plurality of members, each of said members comprising a solid support attached to an oligomer having the formula:-X11-Z-X12 whereinX11 and X12 are complementary oligonucleotides each comprising from 6 to 30 nucleic acid monomers;

1. A synthetic conformationally-restricted probe library comprising a plurality of members, each of said members comprising a solid support attached to an oligomer having the formula:-X11-Z-X12 whereinX11 and X12 are complementary oligonucleotides each comprising from 6 to 30 nucleic acid monomers; each Z is a peptide probe having from about 4 to about 12 amino acids; and X11 and X12 form a double-stranded portion of said member and thereby restrict the conformations available to said peptide probe.