[미국특허]
Single channel, single dilution detection method for the identification and quantification of blood cells and platelets in a whole blood sample using an automated hematology analyzer
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
G01N-033/48
G01N-021/00
출원번호
US-0539568
(2000-03-31)
우선권정보
ter-fluorescence spatial parameters by using, computing and displaying said scattering measurements and fluorescence signals of (); wherein detection and measurement are performed on each of
발명자
/ 주소
Zelmanovic, David
Jones, Valentine
출원인 / 주소
Bayer Corporation
대리인 / 주소
Morgan & Finnegan, LLP
인용정보
피인용 횟수 :
36인용 특허 :
15
초록▼
The invention provides a new single channel, single dilution method and system for identifying, analyzing and quantifying the cellular components of whole blood using a single channel, rather than multiple channels, of an automated hematology analyzer utilizing flow cytometry and the detection of th
The invention provides a new single channel, single dilution method and system for identifying, analyzing and quantifying the cellular components of whole blood using a single channel, rather than multiple channels, of an automated hematology analyzer utilizing flow cytometry and the detection of the light scattered and absorbed by each cell. The single channel utilized in the method was previously known and used only for red blood cell and reticulocyte analysis. The method involves the use of an organic dye in the reagent solution for staining the nucleic acid of reticulocytes, including reticulated platelets, and white blood cells in the sample. The single channel method developed and described is particularly useful for determining white blood cell counts and assessing parameters of a whole blood sample, for blood samples from both human and non-human mammals. The single channel method of the invention employs only one blood diluent reagent composition that is mixed with the blood sample aliquot for analysis and, optimally, a sheath/rinse reagent, thus making it simpler and easier to use. In addition, the reagent compositions of the method are more benign to the white blood cells than previous reagents, which contained various lytic surfactants for removing red blood cells, for example. The apparatus for performing the method provides an economical, streamlined and space-saving analyzer for carrying out complete blood cell analysis on a mammalian whole blood sample.
대표청구항▼
The invention provides a new single channel, single dilution method and system for identifying, analyzing and quantifying the cellular components of whole blood using a single channel, rather than multiple channels, of an automated hematology analyzer utilizing flow cytometry and the detection of th
The invention provides a new single channel, single dilution method and system for identifying, analyzing and quantifying the cellular components of whole blood using a single channel, rather than multiple channels, of an automated hematology analyzer utilizing flow cytometry and the detection of the light scattered and absorbed by each cell. The single channel utilized in the method was previously known and used only for red blood cell and reticulocyte analysis. The method involves the use of an organic dye in the reagent solution for staining the nucleic acid of reticulocytes, including reticulated platelets, and white blood cells in the sample. The single channel method developed and described is particularly useful for determining white blood cell counts and assessing parameters of a whole blood sample, for blood samples from both human and non-human mammals. The single channel method of the invention employs only one blood diluent reagent composition that is mixed with the blood sample aliquot for analysis and, optimally, a sheath/rinse reagent, thus making it simpler and easier to use. In addition, the reagent compositions of the method are more benign to the white blood cells than previous reagents, which contained various lytic surfactants for removing red blood cells, for example. The apparatus for performing the method provides an economical, streamlined and space-saving analyzer for carrying out complete blood cell analysis on a mammalian whole blood sample. " (1984) Proc. Natl. Acad. Sci. (U.S.A.) 81: 3153. Smithies, O. "Insertion of DNA Sequences into the Human Chromosomal β-globin Locus by Homologous Recombination," (1985) Nature 317: 230. Song et al. "Accurate Modification of a Chromosomal Plasmid by Homologous Recombination in Human Cells," (1987) Proc. Natl. Acad. Sci. (U.S.A.) 84: 6820. Doetschman et al. "Targeted Correction of a Mutant HPRT Gene in Mouse Embryonic Stem Cells," (1987) Nature 330: 576. Kim and Smithies "Recombinant Fragment Assay for Gene Targeting Based on the Polymerase Chain Reaction," (1988) Nucleic Acids Res. 16: 8887. Doetschman et al. "Targeted Mutation of the HPRT in Mouse Embryonic Stem Cells," Proc. Natl. Sci. U.S.A., 85(22):8583-7 (1988). Koller and Smithies, "Inactivating the Beta 2-Microglobulin Locus in Mouse Embryonic Stem Cells by Homologous Recombination," Proc. Natl. Acad. Sci. USA, 86(22):8932-5 (1989). Shesely et al. "Correction of a Human βs-Globin Gene by Gene Targeting," (1991) Proc. Natl. Acad. Sci. (U.S.A.) 88: 4294. Kim et al. "Problems Encountered in Detecting a Targeted Gene by the Polymerase Chain Reaction," (1991) Gene 103: 227. Cox and Lehman "Enzymes of General Recombination," (1987) Ann. Rev. Biochem. 56: 229. Radding, C.M. "Homologous Pairing and Strand Exchange in Genetic Recombination," Annu. Rev. Genet. 16:405-37 (1982). Madiraju et al. "Properties of a Mutant recA-Encoded Protein Reveal a Possible Role for Escherichia Coli recF- Encoded Protein in a
Zelmanovic David ; Colella Gregory M. ; Hetherington Edward J. ; Chapman Evelyn Sabrinah ; Paseltiner Lynn, Automated method and device for identifying and quantifying platelets and for determining platelet activation state usi.
Zelmanovic David ; Paseltiner Lynn ; Sorette Martin, Fully automated method and reagent composition therefor for rapid identification and characterization of reticulocytes erythrocytes and platelets in whole blood.
Colella Gregory M. (Bloomfield NJ) Ben-David Daniel (Shrub Oak NY) Cupo Albert (Scarsdale NY) Fan Sophie S. (Millwood NY) Fischer Gena (Harrington Park NJ) Martin Grace E. (Mount Kisco NY) Ornstein L, Methods for the identification and characterization of reticulocytes in whole blood.
Fan Sophie S. (Millwood NY) Ben-David Daniel (Shrub Oak NY) Colella Gregory M. (Bloomfield NJ) Cupo Albert (Scarsdale NY) Fischer Gena (Harrington Park NJ) Ornstein Leonard (White Plains NY), Methods for the identification and characterization of reticulocytes in whole blood.
Ward Anthony J. (San Ramon CA) Mercolino Thomas J. (Pleasanton CA) Recktenwald Diether J. (Cupertino CA), One step method for detection and enumeration of absolute counts of one more cell populations in a sample.
Fan Sophie S. (Millwood NY) Ben-David Daniel (Shrub Oak NY) Cupo Albert (Scarsdale NY) Fischer Gena (Harrington Park NJ) Martin Grace E. (Mount Kisco NY) Ornstein Leonard (White Plains NY) Colella Gr, Reagent compositions and their use in sphering cells.
Fan Sophie S. (Millwood NY) Ben-David Daniel (Shrub Oak NY) Colella Gregory M. (Bloomfield NJ) Cupo Albert (Scarsdale NY) Fischer Gena (Harrington Park NJ) Ornstein Leonard (White Plains NY), Reagent compositions and their use in the identification and characterization of reticulocytes in whole blood.
Colella Gregory M. (Bloomfield NJ) Ben-David Daniel (Shrub Oak NY) Cupo Albert (Scarsdale NY) Fan Sophie S. (Millwood NY) Fischer Gena (Harrington Park NJ) Martin Grace E. (Mount Kisco NY) Ornstein L, Reagent compositions for use in the identification and characterization of reticulocytes in whole blood.
Vacca, Giacomo; Goldblatt, Norman R.; Yee, Michael W., Method and apparatus for rapidly counting and identifying biological particles in a flow stream.
Vacca, Giacomo; Goldblatt, Norman R.; Yee, Michael W., Method and apparatus for rapidly counting and identifying biological particles in a flow stream.
Vacca, Giacomo; Goldblatt, Norman R.; Yee, Michael W., Method and apparatus for rapidly counting and identifying biological particles in a flow stream.
Vacca, Giacomo; Kendall, Richard G.; Goldblatt, Norman R.; Yee, Michael W.; Junnarkar, Mahesh R., Method and apparatus for rapidly counting and identifying biological particles in a flow stream.
Vacca, Giacomo; Kendall, Richard G.; Goldblatt, Norman R.; Yee, Michael W.; Junnarkar, Mahesh R., Method and apparatus for rapidly counting and identifying biological particles in a flow stream.
Battrell,C. Frederick; Shen,Mingchao; Weigl,Bernhard H.; Houkal,Jeffrey M.; Lancaster,Christy A.; Breidford,Wayne, Method and system for microfluidic manipulation, amplification and analysis of fluids, for example, bacteria assays and antiglobulin testing.
Krockenberger, Martin; Wu, Jiong; Roemer, Bodo; Vacca, Giacomo, Method for discriminating red blood cells from white blood cells by using forward scattering from a laser in an automated hematology analyzer.
Krockenberger, Martin; Wu, Jiong; Roemer, Bodo; Vacca, Giacomo, Method for discriminating red blood cells from white blood cells by using forward scattering from a laser in an automated hematology analyzer.
Krockenberger, Martin; Wu, Jiong; Roemer, Bodo; Vacca, Giacomo, Method for discriminating red blood cells from white blood cells by using forward scattering from a laser in an automated hematology analyzer.
Krockenberger, Martin; Bordenkircher, Richard B.; Garrett, Diana G.; Glazier, John G.; Bearden, James C.; Roemer, Bodo; Vacca, Giacomo, Method for flagging a sample.
Zhao, Yang; Lei, Ting; Zhao, Yumei; Xu, Bing, Reagent and kit for classifying and counting leukocytes, the preparation thereof, and process for classifying and counting leukocytes.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.