초록

Described herein are methods and compositions for the production and secretion of polypeptides. Included herein is the use of interrupting peptide transport activity for an increase in polypeptide production and/or secretion.

대표청구항 ▼

1. A method of increasing secretion of a polypeptide in a cell wherein said cell is a member of the genus Bacillus that expresses at least one peptide transport protein comprising: inactivating at least one peptide transport protein in said cell, wherein said peptide transport protein is encoded

1. A method of increasing secretion of a polypeptide in a cell wherein said cell is a member of the genus Bacillus that expresses at least one peptide transport protein comprising: inactivating at least one peptide transport protein in said cell, wherein said peptide transport protein is encoded by an operon selected from the group consisting of the decoyinine inducible (dciA) operon, and the oligonucleotide ABC transporter (opp) operon; and culturing said cell under conditions suitable for expression and secretion of said polypeptide. 2. The method of claim 1 wherein said member of the genus Bacillus is selected from the group consisting of and B. licheniformls, B. lentus, B. brevus, B. stearothermophilus, B. alkalophilus, B. amylotiquefaciens, B. coagulans, B. circulans, B lautus, B. methoanolicus, B. anthracis and B. thuringiensis. 3. The method of claim 1 wherein said polypeptide is selected from the group consisting of hormones, enzymes, growth factors, and cytokines. 4. The method of claim 1 wherein said polypeptide is heterlogous. 5. The method of claim 1 wherein said polypeptide is an enzyme selected from the group consisting of proteases, carbohydrases, reductases, lipases, isomerases, transferases, kinases, phosphatases, cellulase, endoglucosidase H, oxidases, alpha-amylases, glucoamylases, lignocellulose, hemicellulase, pectinase, and ligninase. 6. The method of claim 1 wherein said polypeptide is a Bacillus amylas. 7. The method of claim 1 wherein said polypeptide is subtilisin. 8. The method of claim 1 wherein said polypeptide is amylase. 9. The method of claim 8 wherein said polypeptide is Bacillus amylase. 10. The method of claim 1 wherein said inactivating comprises mutating a nucleic acid encoding said peptide transport protein. 11. The method of claim 10 wherein said mutating causes a frameshift. 12. The method of claim 10 wherein said mutating is deleting one or more nucleotides. 13. A method of producing a polypeptide in a Bacillus cell comprising the steps of: a) obtaining a cell comprising nucleic acid encoding a polypeptide to be produced, said cell further comprising a peptide transport operon wherein at least one gene product of said peptide transport operon is inactive in said cell, and further wherein said peptide transport operon is selected from the group consisting of the decoyinine inducible (dciA) operon, and the oligonucleotide ABC transporter (opp) operon; and b) culturing said cell under conditions suitable for expression such that said polypeptide is produced. 14. The method of claim 13 wherein said polypeptide is heterolgous. 15. The method of claim 13 wherein said gene product is inactive as the result of a mutation in said operon. 16. The method of claim 15 wherein said mutation is a frameshift mutation. 17. The method of claim 15 wherein said mutation is a