초록 ▼

This invention provides a system for producing differentiated cells from a stem cell population for use wherever a relatively homogenous cell population is desirable. The cells contain an effector gene under control of a transcriptional control element (such as the TERT promoter) that causes the gen

This invention provides a system for producing differentiated cells from a stem cell population for use wherever a relatively homogenous cell population is desirable. The cells contain an effector gene under control of a transcriptional control element (such as the TERT promoter) that causes the gene to be expressed in relatively undifferentiated cells in the population. Expression of the effector gene results in depletion of undifferentiated cells, or expression of a marker that can be used to remove them later. Suitable effector sequences encode a toxin, a protein that induces apoptosis, a cell-surface antigen, or an enzyme (such as thymidine kinase) that converts a prodrug into a substance that is lethal to the cell. The differentiated cell populations produced according to this disclosure are suitable for use in tissue regeneration, and non-therapeutic applications such as drug screening.

대표청구항 ▼

This invention provides a system for producing differentiated cells from a stem cell population for use wherever a relatively homogenous cell population is desirable. The cells contain an effector gene under control of a transcriptional control element (such as the TERT promoter) that causes the gen

This invention provides a system for producing differentiated cells from a stem cell population for use wherever a relatively homogenous cell population is desirable. The cells contain an effector gene under control of a transcriptional control element (such as the TERT promoter) that causes the gene to be expressed in relatively undifferentiated cells in the population. Expression of the effector gene results in depletion of undifferentiated cells, or expression of a marker that can be used to remove them later. Suitable effector sequences encode a toxin, a protein that induces apoptosis, a cell-surface antigen, or an enzyme (such as thymidine kinase) that converts a prodrug into a substance that is lethal to the cell. The differentiated cell populations produced according to this disclosure are suitable for use in tissue regeneration, and non-therapeutic applications such as drug screening. ation of IgG in Bovine Milk," J. Agric. Food Chem., 41:682-686 (1993). Lou et al., "One-Step Competitive Immunochromatographic Assay for Semiquantitative Determination of Lipoprotein(a) in Plasma," Clin. Chem., 39:619-624 (1993). Parsons et al., "Multianalyte Assay System Developed for Drugs of Abuse," Clin. Chem., 39:1899-1903 (1993). Rosenzweig et al., "Laser-Based Particle-Counting Microimmunoassay for the Analysis of Single Human Erythrocytes," Anal. Chem., 66:1771-1776 (1994). Reeves et al., "Novel Optical Measurement Approach for the Quantitation of Liposome Immunomigration Assays," Analytical Letters, 28:2347-2352 (1995). Roberts et al., "Investigation of Liposome-Based Immunomigration Sensors for the Detection of Polychlorinated Biphenyls," Analytical Chemistry, 67:482-491 (1995). Siebert et al., "Liposome Immunomigration Field Assay Device for Alachlor Determination," Analytica Chimica Acta, 282:297-305 (1993). Niwa et al., "Electrochemical Behavior of Reversible Redox Species at Interdigitated Array Electrodes with Different Geometries: Consideration of Redox Cycling and Collection Efficiency," Anal. Chem., 62:447-452 (1990). Niwa et al., "Small-Volume Voltammetric Detection of 4-Aminophenol with Interdigitated Array Electrodes and Its Application to Electronchemical Enzyme Immunoassay," Anal. Chem., 65:1559-1563 (1993). Rule et al., "Rapid Method for Visual Identification or Specific DNA Sequences Based on DNA-Tagged Liposomes,"Clin. Chem., 42(8):1206-1209 (1996). Rule et al., "Characteristics of DNA-Tagged Liposomes Allowing Their Use in Capillary-Migration, Sandwich-Hybridization Assays," Anal. Biochem., 244:260-269 (1997). Martorell et al., "Liposome Dehydration on Nitrocellulose and its Application in a Biotin Immunoassay," Analytical Biochemistry 271(2):177-185 (1999). Madden et al., "Protection of Large Unilamellar Vesicles by Trehalose During Dehydration: Retention of Vesicle Contents," Biochim. et Biophys. Acta, 817:67-74 (1985). Crowe et al., "Preservation of Freeze-Dried Liposomes by Trehalose," Archives of Biochem. and Biophys., 242:240-247 (1985). Harrigan et al., "Protection of Liposomes During Dehydration or Freezing," Chemistry and Physics of Lipids, 52:139-149 (1990). Crowe et al., "Preservation of Liposomes by Freeze-Drying," in Liposome Technology, Gregoriadis, ed., CRC Press, Boca Raton, pp. 229-252 (1993). Ausborn et al., "The Protective Effect of Free and Membrane-Bound Cryoprotectants During Freezing and Freeze-Drying of Liposomes," J. of Controlled Release, 30:105-116 (1994). Durst, "Development of a Liposome-Enhanced Assay Format for the Detection of Specific Nucleic Acid Sequences," Cornell Center for Advanced Technology (CAT)--Biotechnology Program, Research Directory (1994-1995) (abstract). Umeda et al., "Liposome Immune Lysis Assay (LILA). Application of Sandwich Method to Determine a Serum Protein Component With Antibody-Bearing Liposomes," J. Immunol. Methods, 95:15-21 (1986). Umeda et al., "A Novel Liposome Immune Lysis Assay (LILA) for Determination of CRP Antigen Using Two Monoclonal Antibodies Recognizing Different Antigenic Determinants," Acta Med. Okayama, 48(6):299-304 (1994). Ho et al., "Interactions of Target-Sensitive Immunoliposomes with Herpes Simplex Virus," J. Biol. Chem., 262(29):13979-13984 (1987). Singh et al., "Application of Antibody and Fluorophore-Derivatized Liposomes to Heterogeneous Immunoassays for D-dimer," Biotechnol. Prog., 12:272-280 (1996). Kung et al., "Large Liposome Agglutinaton Technique for the Serological Detection of Syphilis," J. Immunol. Methods, 90:189-196 (1986).