Stabilized tetrazolium reagent compositions and methods for using the same
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12Q-001/26
C12Q-001/32
C12Q-001/54
출원번호
US-0988812
(2001-11-20)
발명자
/ 주소
Ouyang, Tianmei
Huang, Paing
출원인 / 주소
LifeScan, Inc.
대리인 / 주소
LaSalle, Carol M.Field, Brett E.Bozicevic, Field & Francis, LLP
인용정보
피인용 횟수 :
105인용 특허 :
2
초록▼
Stabilized tetrazolium dye reagent compositions and methods for their use in the measurement of an analyte in a sample are provided. The subject reagent compositions include a tetrazolium dye component, e.g., a water soluble tetrazolium salt, and an effective amount of a nitrite stabilizing agent, e
Stabilized tetrazolium dye reagent compositions and methods for their use in the measurement of an analyte in a sample are provided. The subject reagent compositions include a tetrazolium dye component, e.g., a water soluble tetrazolium salt, and an effective amount of a nitrite stabilizing agent, e.g., a nitrite salt. In many embodiments, the subject reagent compositions include additional members of an analyte oxidizing signal producing system, such as: an analyte oxidizing enzyme, e.g., an analyte dehydrogenase or an analyte oxidase; an electron transfer agent; and an enzyme cofactor. Also provided are test strips that include the subject reagent compositions, as well as systems and kits incorporating the subject test strips. The subject reagent compositions, test strips, systems and kits find use in the detection of a wide variety of analytes in a sample, such as a physiological sample, e.g., blood or a fraction thereof, or ISF (interstitial fluid).
대표청구항▼
Stabilized tetrazolium dye reagent compositions and methods for their use in the measurement of an analyte in a sample are provided. The subject reagent compositions include a tetrazolium dye component, e.g., a water soluble tetrazolium salt, and an effective amount of a nitrite stabilizing agent, e
Stabilized tetrazolium dye reagent compositions and methods for their use in the measurement of an analyte in a sample are provided. The subject reagent compositions include a tetrazolium dye component, e.g., a water soluble tetrazolium salt, and an effective amount of a nitrite stabilizing agent, e.g., a nitrite salt. In many embodiments, the subject reagent compositions include additional members of an analyte oxidizing signal producing system, such as: an analyte oxidizing enzyme, e.g., an analyte dehydrogenase or an analyte oxidase; an electron transfer agent; and an enzyme cofactor. Also provided are test strips that include the subject reagent compositions, as well as systems and kits incorporating the subject test strips. The subject reagent compositions, test strips, systems and kits find use in the detection of a wide variety of analytes in a sample, such as a physiological sample, e.g., blood or a fraction thereof, or ISF (interstitial fluid). in to aminopeptidase in susceptible and resistant diamondback moths (Plutella xylostella)," Appl. Environ. Microbiol. 63:1024-1027. Luo, K., McLachlin, J.R., Brown, M.R., Adang, M.J. (1999) "Expression of a Glycosylphosphatidylinositol-Linked Manduca sexta Aminopeptidase N in Insect Cells," Protein Expression and Purification 17:113-122. Masson, L., Lu, Y., Mazza, Brosseau, R. and Adang, M.J. (1995) "The CryIA(c) receptor purified from Manduca sexta displays multiple specificities," J. Biol. Chem. 270:20309-20315. McConville, M. J. and Ferguson, M. A. J. (1993) "The structure, biosynthesis and function of glycosylated phosphatidylinositols in the parasitic protozoa and higher eukaryotes," Biochemical Journal 294:305-324. McLaughlin, S. and Aderem, A. (1995) "The myristoyl-electrostatic switch: a modulator of reversible protein-membrane interactions," TIBS 20:272-276. Morris, T.D. and Miller, L.K. (1992) "Promoter influence on baculovirus-mediated gene expression in permissive and non-permissive insect cell lines," J. Virol. 66:7397-7405. Plakidou-dymock, S., Tanner, M.J. and McGivan, J.D. (1993) "A role for aminopeptidase N in Na+-dependent amino acid transport in bovine renal brush-border membranes," Biochem. J. 290:59-65. Richardson, M.A., Smith, D.R.J., Kemp, D.H. and Tellam, R.L. (1993) "Native and baculovirus-expressed forms of the immunoprotective protein BM86 from boophilus microplus are anchored to the cell membrane by a glycosylphosphatidyl inositol linkage," Insect Mol. Biol. 1:139-147. Sangadala, S., Walters, F., English, L.H. and Adang, M.J. (1994) "A mixture of Manduca sexta aminopeptidase and alkaline phosphatase enhances Bacillus thuringiensis insecticidal CryIA(c) toxin binding and 86Rb+-K+leakage in Vitro," J. Biol. Chem. 269:10088-10092. Schwartz, J.-L., Lu, Y.J., Soehnlein, P., Brosseau, R., Masson, L., Laprade, R. and Adang, M.J. (1997) "Ion channels formed in planar lipid bilayers by Bacillus thuringiensis toxins in the presence of Manduca sexta midgut receptors," FEBS Lett. 412:270-276. Takasaki, S., Erickson, R.H., Kim, Y.S., Kochibe, N. and Kobata, A. (1991) "N-linked neutral sugar chains of aminopeptidase N purified from rat small intestinal brush-border membrane," Biochem. 30:9102-9110. Tomita, M., Obara, H., Takesue, Y., Tamura, H.-O., Miyajima, s., Taguchi, R. and Ikezawa, H. (1994) "Purification of glycosylphosphatidylinositol-anchoring aminopeptidase N from the plasma membrane of larval midgut epithelial cells of the silkworm, Bombyx mori," Int. J. Biochem. 26:977-986. Towbin, H., Staehelin, T. and Gordon, J. (1979) "Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications," Proc. Natl. Acad. Sci. USA 76:4350-4354. Tresnan, N.B., Levis, R. and Holmes, K.V. (1996) "Feline aminopeptidase N serve as a receptor for feline, canine, porcine, and human coronaviruses in serogroup I," J. Virol. 70:8669-8674. Valaitis, A., Lee, M.K., Rajamohan, F. and Dean, D.H. (1995) "Brush border membrane aminopeptidase-N in the midgut of the gypsy moth serves as the receptor for the CryIA(c) d-endotoxin of Bacillus thuringiensis," Insect Biochem. Molec. Biol. 25:1143-1151. Vaughn, J.L., Goodwin, R.H., Tompkins, G.L. and McCawley, P. (1977) "The establishment of two insect cell lines from the insect Spodoptera frugiperda (Lepidoptera:Noctuidae)," In Vitro Cell. Dev. Biol. 13:213-217. Wolfersberger, M.G., Luthy, P., Maurer, A., Parenti, P
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