Enzymatic nucleic acids containing 5'-and or 3'-cap structures
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C07H-021/04
C12N-005/00
출원번호
US-0419125
(1999-10-15)
발명자
/ 주소
Matulic-Adamic, Jasenka
Beigelman, Leonid
Karpeisky, Alexander
Jarvis, Thale
Usman, Nassim
DiRenzo, Anthony
Wincott, Francine
출원인 / 주소
Ribozyme Pharmaceuticals, Inc.
대리인 / 주소
McDonnell Boehnen Hulbert & Berghoff
인용정보
피인용 횟수 :
14인용 특허 :
14
초록
An enzymatic nucleic acid molecule comprising a 5'- and/or a 3'-cap structure, wherein said structure is not a 5'-5'-linked inverted nucleotide or a 3'-3'-linked inverted nucleotide.
대표청구항▼
1. A nucleic acid molecule comprising a 5'-cap structure, a 3'-cap structure, or both a 5'- and a 3'-cap structure, wherein said 5'-cap structure is selected from the group consisting of 4',5'-methylene nucleotide; 1-(β-D-erythrofuranosyl) nucleotide; 1,3-diamino-2-propyl phosphate, 3-aminopropyl ph
1. A nucleic acid molecule comprising a 5'-cap structure, a 3'-cap structure, or both a 5'- and a 3'-cap structure, wherein said 5'-cap structure is selected from the group consisting of 4',5'-methylene nucleotide; 1-(β-D-erythrofuranosyl) nucleotide; 1,3-diamino-2-propyl phosphate, 3-aminopropyl phosphate; 12-aminododecyl phosphate; hydroxypropyl phosphate; 1,5-anhydrohexitol nucleotide; L-nucleotide; threo-pentofuranosyl nucleotide; acyclic 3',4'-seco nucleotide; 3,5-dihydroxypentyl nucleotide; and 5'-mercapto moieties, and wherein said 3'-cap structure is selected from the group consisting of 1,5-anhydrohexitol nucleotide; L-nucleotide; threo-pentofuranosyl nucleotide; acyclic 3',4'-seco nucleotide; 3,5-dihydroxypentyl nucleotide; 3'-2'-inverted nucleotide moiety; 3'-2'-inverted abasic moiety; and 1,4-butanediol. 2. The nucleic acid molecule of claim 1, wherein said nucleic acid molecule is in an enzymatic nucleic acid molecule. 3. The nucleic acid molecule of claim 1, wherein said nucleic acid molecule is in an antisense nucleic acid molecule. 4. The nucleic acid molecule of claim 2, wherein said enzymatic nucleic acid molecule is in a hairpin, hepatitis delta virus, group I intron, VS RNA or RNase P RNA motif. 5. The nucleic acid molecule of claim 1, wherein said nucleic acid molecule comprises said 5'-cap structure. 6. The nucleic acid molecule of claim 1, wherein said nucleic acid molecule comprises said 3'-cap structure. 7. The nucleic acid molecule of claim 1, wherein said nucleic acid molecule comprises said 5'-cap structure and said 3'-cap structure. 8. The nucleic acid molecule of claim 1, wherein said 5'- and 3'-cap structures are different. 9. The nucleic acid molecule of claim 1, wherein said 5'- and 3'-cap structures are same. 10. The nucleic acid of claim 1, wherein said 3'-cap structure is a 3'-2' linked inverted nucleotide. 11. The nucleic acid of claim 1, wherein said 3'-cap structure is a 3'-2' linked inverted abasic moiety. 12. The nucleic acid of claim 1, wherein said 5'-cap structure is a 1,3-diamino-2propyl phosphate group. 13. The nucleic acid of claim 1, wherein said 5'-cap structure is a L-nucleotide. 14. The nucleic acid of claim 1, wherein said 5'-cap structure is a threo-pentafuranosyl group. 15. The nucleic acid of claim 1, wherein said 5'-cap structure is a 3,5-dihydroxypentyl nucleotide. 16. The nucleic acid of claim 1, wherein said 5'-cap structure is a 1-(β-D-erythrofuranosyl) nucleotide. 17. The nucleic acid of claim 1, wherein said 3'-cap structure is a L-nucleotide. 18. The nucleic acid of claim 1, wherein said 3'-cap structure is a 3,5-dihydroxypentyl nucleotide. 19. An isolated mammalian cell comprising the nucleic acid molecule of claim 1. 20. The mammalian cell of claim 19, wherein said mammalian cell is a human cell.
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이 특허에 인용된 특허 (14)
Beigelman Leonid (Longmont CO) Karpeisky Alex (Boulder CO), Base-modified enzymatic nucleic acid.
Asgari Morteza (Houston TX) Blick Mark (Houston TX) Bresser Joel (Bellaire TX) Cubbage Michael L. (Houston TX) Prashad Nagindra (Houston TX), Enriching and identifying fetal cells in maternal blood for in situ hybridization.
Torrence Paul (Silver Spring MD) Silverman Robert (Shaker Heights OH) Maitra Ratan (Euclid OH) Lesiak Krystyna (Gaithersburg MD), Method of cleaving specific strands of RNA.
Letsinger Robert L. (Wilmette IL) Gryaznov Sergei M. (San Mateo CA), Oligodeoxyribonucleotides including 3′-aminonucleoside-phosphoramidate linkages and terminal 3′-amino groups.
Cech Thomas R. (Boulder CO) Zaug Arthur J. (Louisville CO) Been Michael D. (Boulder CO), RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods.
Trulson, Mark O.; McGall, Glenn H.; Sywe, Bei-Shen; Kajisa, Lisa T.; Truong, Dana; Rava, Rich P.; Goldberg, Martin J., Antireflective coatings for high-resolution photolithographic synthesis of DNA array.
Trulson, Mark O.; McGall, Glenn H.; Sywe, Bei-Shen; Kajisa, Lisa T.; Truong, Dana; Rava, Richard P.; Goldberg, Martin J., Antireflective coatings for high-resolution photolithographic synthesis of DNA arrays.
Trulson, Mark O.; McGall, Glenn H.; Sywe, Bei-Shen; Kajisa, Lisa T.; Truong, Dana; Rava, Richard P.; Goldberg, Martin J., Antireflective coatings for high-resolution photolithographic synthesis of DNA arrays.
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