초록 ▼

The invention provides a method of separating from corn fiber a lipid fraction having phytosterol esters and phytosterols where the method has the steps of: (a) heating an aqueous mixture of unground corn fiber; (b) contacting the mixture of step (a) with at least one enzyme suitable for digesting s

The invention provides a method of separating from corn fiber a lipid fraction having phytosterol esters and phytosterols where the method has the steps of: (a) heating an aqueous mixture of unground corn fiber; (b) contacting the mixture of step (a) with at least one enzyme suitable for digesting starch for a time and at a temperature suitable to provide a mixture of an essentially destarched corn fiber and a liquid comprising soluble carbohydrates; (c) contacting the mixture of step (a) or (b) with a protease enzyme to provide a proteolyzed corn fiber and a liquid; (d) separating the liquid of step (c) from the corn fiber to provide a destarched, proteolyzed corn fiber; and (e) extracting the destarched, proteolyzed corn fiber with at least one organic solvent. A corn fiber lipid fraction/organic solvent solution having phytosterol esters and phytosterols is provided. The invention also provides a corn fiber lipid fraction containing phytosterols and phytosterol esters obtained via solvent extraction of a proteolyzed corn fiber. The concentration of phytosterols and phytosterol esters in the lipid fraction is at least about 1.4 times greater than the concentration of phytosterols and phytosterol esters in the lipid fraction of a nonproteolyzed corn fiber.

대표청구항 ▼

The invention provides a method of separating from corn fiber a lipid fraction having phytosterol esters and phytosterols where the method has the steps of: (a) heating an aqueous mixture of unground corn fiber; (b) contacting the mixture of step (a) with at least one enzyme suitable for digesting s

The invention provides a method of separating from corn fiber a lipid fraction having phytosterol esters and phytosterols where the method has the steps of: (a) heating an aqueous mixture of unground corn fiber; (b) contacting the mixture of step (a) with at least one enzyme suitable for digesting starch for a time and at a temperature suitable to provide a mixture of an essentially destarched corn fiber and a liquid comprising soluble carbohydrates; (c) contacting the mixture of step (a) or (b) with a protease enzyme to provide a proteolyzed corn fiber and a liquid; (d) separating the liquid of step (c) from the corn fiber to provide a destarched, proteolyzed corn fiber; and (e) extracting the destarched, proteolyzed corn fiber with at least one organic solvent. A corn fiber lipid fraction/organic solvent solution having phytosterol esters and phytosterols is provided. The invention also provides a corn fiber lipid fraction containing phytosterols and phytosterol esters obtained via solvent extraction of a proteolyzed corn fiber. The concentration of phytosterols and phytosterol esters in the lipid fraction is at least about 1.4 times greater than the concentration of phytosterols and phytosterol esters in the lipid fraction of a nonproteolyzed corn fiber. dged cycloalkyl group; Q is --NO or --NO2; and T is independently a covalent bond, a carbonyl, an oxygen, --S(O)o-- or --N(Ra)Ri--, wherein o is an integer from 0 to 2, Rais a lone pair of electrons, a hydrogen or an alkyl group; Riis a hydrogen, an alkyl, an aryl, an alkylcarboxylic acid, an aryl carboxylic acid, an alkylcarboxylic ester, an arylcarboxylic ester, an alkylcarboxamido, an arylcarboxamido, an alkylaryl, an alkylsulfinyl, an alkylsulfonyl, an arylsulfinyl, an arylsulfonyl, a sulfonamido, a carboxamido, a carboxylic ester, an amino alkyl, an amino aryl, --CH2--C(T--Q)(Re)(Rf), or --(N2O2-)-·M+,wherein M+is an organic or inorganic cation; with the proviso that when Riis --CH2--C(T--Q)(Re)(Rf) or --(N2O2-)·M+; then "--T--Q" can be a hydrogen, an alkyl group, an alkoxyalkyl group, an aminoalkyl group, a hydroxy group or an aryl group. 6. The composition of claim 1, wherein the at least one compound that donates, transfers, or releases nitric oxide, or induces the production of endogenous nitric oxide or endothelium-derived relaxing factor, or is a substrate for nitric oxide synthase is: (i) a compound that comprises at least one ON--O--, ON--N-- or ON--C-- group; (ii) a compound that comprises at least one O2N--O--, O2N--N--, O2N--S-- or --O2N--C-- group; (iii) a N-oxo-N-nitrosoamine having the formula: R1R2--N(O--M+)--NO, wherein R1and R2are each independently a polypeptide, an amino acid, a sugar, an oligonucleotide, a straight or branched, saturated or unsaturated, aliphatic or aromatic, substituted or unsubstituted hydrocarbon, or a heterocyclic group, and M+is an organic or inorganic cation. 7. The composition of claim 6, wherein the compound comprising at least one ON--O--, ON--N-- or ON--C-- group is an ON--O-polypeptide, an ON--N-polypeptide, an ON--C-polypeptide, an ON--O-amino acid, an ON--N-amino acid, an ON--C-amino acid, an ON--O-sugar, an ON--N-sugar, an ON--C-sugar, an ON--O-oligonucleotide, an ON--N-oligonucleotide, an ON--C-oligonucleotide, a straight or branched, saturated or unsaturated, substituted or unsubstituted, aliphatic or aromatic ON--O-hydrocarbon, a straight or branched, saturated or unsaturated, substituted or unsubstituted, aliphatic or aromatic ON--N-hydrocarbon, a straight or branched, saturated or unsaturated, substituted or unsubstituted, aliphatic or aromatic ON--C-hydrocarbon, an ON--O-heterocyclic compound, an ON--N-heterocyclic compound or a ON--C-heterocyclic compound. 8. The composition of claim 6, wherein compound comprising at least one O2N--O--, O2N--N--, O2N--S-- or O2N--C-- group is an O2N--O-polypeptide, an O2N--N-polypeptide, an O2N--S-polypeptide, an O2N--C-polypeptide, an O2N--O-amiino acid, O2N--N-amino acid, O2N--S-amino acid, an O2N--C-amino acid, an O2N--O-sugar, an O2N--N-sugar, O2N--S-sugar, an O2N--C-sugar, an O2N--O -oligonucleotide, an O2N--N-oligonucleotide, an O2N--S-oligonucleotide, an O2N--C-oligonucleotide, a straight or branched, saturated or unsaturated, aliphatic or aromatic, substituted or unsubstituted O2N--O-hydrocarbon, a straight or branched, saturated or unsaturated, aliphatic or aromatic, substituted or unsubstituted O2N--N-hydrocarbon, a straight or branched, saturated or unsaturated, aliphatic or aromatic, substituted or unsubstituted O2N--S-hydrocarbon, a straight or branched, saturated or unsaturated, aliphatic or aromatic, substituted or unsubsti