IPC분류정보
국가/구분 |
United States(US) Patent
등록
|
국제특허분류(IPC7판) |
|
출원번호 |
US-0019763
(1700-01-01)
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국제출원번호 |
PCT/US01/11701
(2001-04-09)
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발명자
/ 주소 |
- Kelly, Craig A.
- Murray, George M.
- Uy, O. Manuel
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출원인 / 주소 |
- The Johns Hopkins University
|
대리인 / 주소 |
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인용정보 |
피인용 횟수 :
9 인용 특허 :
4 |
초록
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A polymeric food spoilage sensor comprises a polymer containing a polyazamacrocyclic transition metal complex. The complex selectively binds biogenic amines, such as cadaverine, putrescine and histamine, which are released by food spoilage microorganisms. The polymer undergoes a detectable color cha
A polymeric food spoilage sensor comprises a polymer containing a polyazamacrocyclic transition metal complex. The complex selectively binds biogenic amines, such as cadaverine, putrescine and histamine, which are released by food spoilage microorganisms. The polymer undergoes a detectable color change upon exposure to biogenic amine, thus indicating that food spoilage has probably occurred. In one embodiment, the polymer is molecularly imprinted with the biogenic amine to impart selective binding affinity. The polymer is easily incorporated in common food containers and can be employed in fiber optic detection devices.
대표청구항
▼
A polymeric food spoilage sensor comprises a polymer containing a polyazamacrocyclic transition metal complex. The complex selectively binds biogenic amines, such as cadaverine, putrescine and histamine, which are released by food spoilage microorganisms. The polymer undergoes a detectable color cha
A polymeric food spoilage sensor comprises a polymer containing a polyazamacrocyclic transition metal complex. The complex selectively binds biogenic amines, such as cadaverine, putrescine and histamine, which are released by food spoilage microorganisms. The polymer undergoes a detectable color change upon exposure to biogenic amine, thus indicating that food spoilage has probably occurred. In one embodiment, the polymer is molecularly imprinted with the biogenic amine to impart selective binding affinity. The polymer is easily incorporated in common food containers and can be employed in fiber optic detection devices. 1-II), and phasins from R. eutropha (P1-I and P1-II). 12. The bacterium of claim 1 wherein the integrated gene is operably linked as a single copy on the chromosome of the microorganism. 13. A method for screening for a gene involved in synthesis of dipolyhydroxyalkanoates that enhances production comprising mutating genetically engineered bacterium having at least one heterologous gene encoding at least one enzyme involved in synthesis of polyhydroxyalkanoates selected from the group consisting of beta-ketothiolase, acetoacetyl-CoA reductase, polyhydroxyalkanoate synthase, acyl-CoA transferase, and enoyl-CoA hydratase, wherein the heterologous gene is integrated into the chromosome, which stably expresses the enzyme and thereby has enhanced production of polyhydroxyalkanoates, and culturing the bacterium with appropriate substrate under conditions wherein bacterial colonies form and polyhydroxyalkanoate is produced; and screening for enhanced production of polyhydroxyalkanoates, wherein the enhanced production of polyhydroxyalkanoate is evident by an increase in opacity of the bacterial colony. 14. The method of claim 13 wherein the gene encodes an enzyme selected from the group consisting of A. eutrophus, Aeromhonas caviae, Zoogloea ramigera, Nocardia, Rhodococcus, Pseudomonas Sp. 61-3, Pseudomonas acidophila, Pseudomonas oleovarans, Chromobacterium violaceum, and Alcaligenes lactus. 15. The method of claim 13 wherein one or more endogenous genes encoding an enzyme or enzymes required for production of polyhydroxyalkanoates are missing or encode an inactive enzyme or enzymes. 16. The method of claim 13 wherein the bacteria produce polyhydroxyalkanoates, further comprising selecting genes which result in increased polyhydroxyalkanoate production. 17. A method for producing polyhydroxyalkanoates comprising culturing genetically engineered bacteria having at least one gene encoding an enzyme involved in synthesis of polyhydroxyalkanoates selected from the group consisting of thiolase, reductase, PHB synthase, PHA synthase, acyl-CoA transferase, enoyl-CoA hydratase, integrated into the chromosome, with appropriate substrate under conditions wherein the bacteria produce polyhydroxyalkanoate. 18. The method of claim 17 wherein the bacteria comprise multiple genes involved in synthesis of polyhydroxyalkanoate wherein the integrated genes are operably linked as an operon. 19. The method of claim 17 wherein the bacteria comprise genes selected from the group consisting of genes integrated operably linked under the control of a promoter, genes integrated and operably linked with upstream activating sequences, genes integrated and operably linked with mRNA stabilizing sequences, genes operably linked with a promoter including a consensus E. coli pho box and -35 promoter region that is regulated by the phosphate concentration in the medium, and genes integrated and operably linked with a selection marker. erculosis from the complete genome sequence", Nature, vol. 393, pp. 537-544, Jun. 1, 1998. P. Mantsala et al., "Cloning and Sequence of Bacillus subtilis purA and guaA, Involved in the Conversion of IMP to AMP and GMP", vol. 174(6) Journal of Bacteriology, Mar. (1992), pp. 1883-1890. i (Bangalore) 11:47-58 (1987). Sloane, B.F. et al. (Proc. Natl. Acad. Sci. USA 83:2483-2487 (1986). Spiess, E. et al., J. Histochem. Cytochem. 42:917-929 (1994). 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